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Literature summary extracted from

  • Faust, C.; Ye, B.; Song, K.
    The enzymatic domain of Clostridium difficile toxin A is located within its N-terminal region (1998), Biochem. Biophys. Res. Commun., 251, 100-105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B62 expression in Escherichia coli Clostridioides difficile

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B62 additional information construction of truncation mutants. The enzymatic domain for binding UDP-glucose, for catalytically transferring glucose to Rho A and for recognizing the interaction interface of Rho A resides in the first N-terminal 659 amino acids of toxin A Clostridioides difficile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.B62 308000
-
 x * 308000, calculated Clostridioides difficile

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B62 additional information Clostridioides difficile enzyme glucosylates and inactivates small GTPases of the Rho or Ras families, culminating in cytotoxicity ?
-
 ?
2.4.1.B62 UDP-alpha-D-glucose + RhoA Clostridioides difficile
-
 UDP + D-glucosyl-RhoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B62 Clostridioides difficile P16154
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B62 additional information enzyme glucosylates and inactivates small GTPases of the Rho or Ras families, culminating in cytotoxicity Clostridioides difficile ?
-
 ?
2.4.1.B62 UDP-alpha-D-glucose + RhoA
-
 Clostridioides difficile UDP + D-glucosyl-RhoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B62 ? x * 308000, calculated Clostridioides difficile

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B62 toxA
-
 Clostridioides difficile
2.4.1.B62 toxin A
-
 Clostridioides difficile

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B62 30
-
 assay at Clostridioides difficile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B62 7.5
-
 assay at Clostridioides difficile