EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.1 | expression in Escherichia coli | Methanothermobacter thermautotrophicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.2.1.1 | A498A | alteration does not significantly affect the Km for any substrate but reduces the turnover rate kcat 41fold | Methanothermobacter thermautotrophicus |
6.2.1.1 | A500T | kcat value decreases 44fold | Methanothermobacter thermautotrophicus |
6.2.1.1 | D502A | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
6.2.1.1 | D502E | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
6.2.1.1 | D502N | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
6.2.1.1 | G501A | two- to threefold reduced Km values for acetate, ATP and CoA. The turnover rate is over 200fold reduced | Methanothermobacter thermautotrophicus |
6.2.1.1 | T499A | kcat value decreases 83fold | Methanothermobacter thermautotrophicus |
6.2.1.1 | Y498F | inactive mutant enzyme | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.1 | 0.08 | - |
CoA | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.1 | - |
CoA | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.19 | - |
CoA | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.24 | - |
CoA | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.5 | 1 | CoA | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.4 | - |
acetate | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.7 | - |
ATP | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.7 | - |
ATP | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.7 | - |
ATP | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 2.1 | - |
acetate | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 3 | - |
acetate | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 3.3 | - |
ATP | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 3.5 | - |
acetate | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 3.6 | - |
ATP | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 7.5 | - |
acetate | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.1 | Methanothermobacter thermautotrophicus | Q2I0L7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.1 | - |
Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.1 | ATP + acetate + CoA | important role for motif III (498YTAGD502) in catalysis. The highly conserved Tyr in the first position may play a key role in active-site architecture through interaction with a highly conserved active-site Gln. The invariant Asp in the fifth position plays a critical role in ATP binding and catalysis through interaction with the 2'- and 3'-OH groups of the ribose moiety of ATP | Methanothermobacter thermautotrophicus | AMP + diphosphate + acetyl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.1 | ACS1 | - |
Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 65 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.1 | 0.3 | - |
ATP | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.3 | - |
CoA | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.3 | - |
acetate | pH 7.0, 65°C, mutant enzyme G501A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.8 | - |
ATP | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.8 | - |
acetate | pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 0.8 | - |
CoA | pH pH 7.0, 65°C, mutant enzyme T499A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.5 | - |
ATP | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.5 | - |
CoA | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.5 | - |
acetate | pH 7.0, 65°C, mutant enzyme A500T | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.6 | - |
ATP | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.6 | - |
CoA | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 1.6 | - |
acetate | pH 7.0, 65°C, mutant enzyme Y498A | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 66.6 | - |
ATP | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 66.6 | - |
CoA | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
6.2.1.1 | 66.6 | - |
acetate | pH 7.0, 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 7 | 7.5 | assay at | Methanothermobacter thermautotrophicus |