Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, Z.; Zhu, L.; Xu, J.; Mon, H.; Lee, J.M.; Kusakabe, T.
    Amino Acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells (2013), Arch. Insect Biochem. Physiol., 83, 57-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 from BmN4 cells, recombinant expression of the GFP-tagged or Venus fluorescent protein-tagged enzyme in BmN4 cells Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.4 additional information RNA interference is used to silence BmASNS expression in silkworm cells Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.4 62800
-
 x * 62800, about, sequence calculation Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine + H2O Bombyx mori
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Bombyx mori Q2L9S5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 BmN4 cell
-
 Bombyx mori
-
6.3.5.4 BmN4-SID1 cell the cell line expresses a Caenorhabditis elegans SID-1 (CeSID-1) transmembrane protein with the ability to uptake double-stranded RNA into the cells Bombyx mori
-
6.3.5.4 cell culture
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine + H2O
-
 Bombyx mori AMP + diphosphate + L-asparagine + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.4 ? x * 62800, about, sequence calculation Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 ASNS
-
 Bombyx mori
6.3.5.4 Asparagine synthetase
-
 Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.4 ATP
-
 Bombyx mori

General Information

EC Number General Information Comment Organism
6.3.5.4 malfunction silencing of the BmASNS gene enhances the sensitivity of silkworm cells to amino acid starvation. Ectopic overexpression of BmASNS gene effectively inhibits cell growth in silkworm cells, whereas its overexpression can rescue cell growth upon amino acid deprivation treatment. Silkworm cells lacking BmASNS under the condition of amino acid deprivation show severely impaired proliferation Bombyx mori
6.3.5.4 physiological function BmAsns protein negatively regulates silkworm cell proliferation. The recovery of cell growth by overexpressed BmAsns protein is due to the rapid turnover of autophagic vacuoles in the cells Bombyx mori