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Literature summary extracted from
- A novel activity for fungal nitronate monooxygenase: detoxification of the metabolic inhibitor propionate-3-nitronate (2012), Arch. Biochem. Biophys., 521, 84-89.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.16 | 0.27 | - |
propionate-3-nitronate | pH 7.4, 30°C | Cyberlindnera saturnus |  |
| 1.13.12.16 | 0.34 | - |
propionate-3-nitronate | pH 7.4, 30°C | Neurospora crassa | |
| 1.13.12.16 | 0.58 | - |
3-nitropropionate | pH 7.4, 30°C | Neurospora crassa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.16 | Cyberlindnera saturnus | - |
- |
- |
| 1.13.12.16 | Neurospora crassa | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.16 | 3-nitropropionate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | propionate-3-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | propionate-3-nitronate + O2 | - |
Cyberlindnera saturnus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.12.16 | nitronate monooxygenase | - |
Neurospora crassa |
| 1.13.12.16 | nitronate monooxygenase | - |
Cyberlindnera saturnus |
| 1.13.12.16 | NMO-Nc | - |
Neurospora crassa |
| 1.13.12.16 | NMO-Ws | - |
Cyberlindnera saturnus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.16 | 30 | - |
assay at | Neurospora crassa |
| 1.13.12.16 | 30 | - |
assay at | Cyberlindnera saturnus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.16 | 4.4 | - |
3-nitropropionate | pH 7.4, 30°C | Neurospora crassa | |
| 1.13.12.16 | 430 | - |
propionate-3-nitronate | pH 7.4, 30°C | Neurospora crassa | |
| 1.13.12.16 | 775 | - |
propionate-3-nitronate | pH 7.4, 30°C | Cyberlindnera saturnus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.16 | 7.4 | - |
assay at | Neurospora crassa |
| 1.13.12.16 | 7.4 | - |
assay at | Cyberlindnera saturnus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.12.16 | malfunction | a knockout mutant of Neurospora crassa lacking NMO is inhibited by concentrations of propionate-3-nitronate and 3-nitropropionate | Neurospora crassa |
| 1.13.12.16 | malfunction | the mixture propionate-3-nitronate/3-nitropropionate is toxic to recombinant Escherichia coli cells lacking expression of NMO, but the toxicity is overcome through either induction of the gene for NMO or through addition of exogenous enzyme to the cultures | Neurospora crassa |
| 1.13.12.16 | malfunction | the mixture propionate-3-nitronate/3-nitropropionate is toxic to recombinant Escherichia coli cells lacking expression of NMO, but the toxicity is overcome through either induction of the gene for NMO or through addition of exogenous enzyme to the cultures | Cyberlindnera saturnus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.16 | 7.6 | - |
3-nitropropionate | pH 7.4, 30°C | Neurospora crassa | |
| 1.13.12.16 | 1300 | - |
propionate-3-nitronate | pH 7.4, 30°C | Neurospora crassa | |
| 1.13.12.16 | 2800 | - |
propionate-3-nitronate | pH 7.4, 30°C | Cyberlindnera saturnus | |
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