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Literature summary extracted from

  • Zhao, B.; Bellamine, A.; Lei, L.; Waterman, M.
    The role of Ile87 of CYP158A2 in oxidative coupling reaction (2012), Arch. Biochem. Biophys., 518, 127-132.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.69 expressed in Escherichia coli BL21 (DE3) pLysS cells Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.19.69 I87K mutant of isoform CYP158A2, hanging drop vapor diffusion method, using 0.1 M bis-Tris (pH 6.5), 1.2 M ammonium dihydrogen phosphate Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.69 I87K mutant of isoform CYP158A2, the mutation significantly changes the ratios of the dimerization products converting CYP158A2 into a CYP158A1-like monooxygenase Streptomyces coelicolor
1.14.19.69 K90I mutant of isoform CYP158A1, the catalytic activity of the mutant does not change at all compared with the wild type enzyme Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.69 0.0073
-
 flaviolin wild type isoform CYP158A2, at pH 8.2 and 37°C Streptomyces coelicolor
1.14.19.69 0.0105
-
 flaviolin wild type isoform CYP158A1, at pH 8.2 and 37°C Streptomyces coelicolor
1.14.19.69 0.0169
-
 flaviolin mutant K90I of isoform CYP158A1, at pH 8.2 and 37°C Streptomyces coelicolor
1.14.19.69 0.0432
-
 flaviolin mutant I87K of isoform CYP158A2, at pH 8.2 and 37°C Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 Streptomyces coelicolor CYP158A2 produces three isomers of biflaviolin and one triflaviolin 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 Streptomyces coelicolor isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 Streptomyces coelicolor A3(2) CYP158A2 produces three isomers of biflaviolin and one triflaviolin 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 Streptomyces coelicolor A3(2) isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.69 Streptomyces coelicolor Q9FCA6
-
-
1.14.19.69 Streptomyces coelicolor Q9KZF5
-
-
1.14.19.69 Streptomyces coelicolor A3(2) Q9FCA6
-
-
1.14.19.69 Streptomyces coelicolor A3(2) Q9KZF5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.69 Ni2+ affinity chromatography and S-Sepharose/Q-Sepharose column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 CYP158A2 produces three isomers of biflaviolin and one triflaviolin Streptomyces coelicolor 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 Streptomyces coelicolor 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 CYP158A2 produces three isomers of biflaviolin and one triflaviolin Streptomyces coelicolor A3(2) 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?
1.14.19.69 4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 Streptomyces coelicolor A3(2) 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.69 CYP158A1 isoform Streptomyces coelicolor
1.14.19.69 CYP158A2 isoform Streptomyces coelicolor