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Literature summary extracted from

  • Park, H.G.; Lee, I.S.; Chun, Y.J.; Yun, C.H.; Johnston, J.B.; Montellano, P.R.; Kim, D.
    Heterologous expression and characterization of the sterol 14alpha-demethylase CYP51F1 from Candida albicans (2011), Arch. Biochem. Biophys., 509, 9-15.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expressed in Escherichia coli DH5alpha cells Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.154 D116E the azole Kd value of the mutant enzyme is little altered compared to the wild type enzyme Candida albicans
1.14.14.154 F105L the azole Kd value of the mutant enzyme is little altered compared to the wild type enzyme Candida albicans
1.14.14.154 R467K the azole Kd value of the mutant enzyme is little altered compared to the wild type enzyme Candida albicans
1.14.14.154 Y132H the azole Kd value of the mutant enzyme is clearly altered compared to the wild type enzyme Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 econazole
-
Candida albicans
1.14.14.154 fluconazole
-
Candida albicans
1.14.14.154 itraconazole
-
Candida albicans
1.14.14.154 ketoconazole
-
Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.154 61500
-
x * 61500, SDS-PAGE Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Candida albicans P10613
-
-
1.14.14.154 Candida albicans ATCC MYA-2876 P10613
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.154 Ni-NTA column chromatography, and gel filtration Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
Candida albicans 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
Candida albicans ATCC MYA-2876 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.154 ? x * 61500, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51F1
-
Candida albicans
1.14.14.154 lanosterol 14alpha-demethylase
-
Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.154 heme
-
Candida albicans