BRENDA - Enzyme Database

Eubacteria-type isocitrate dehydrogenase from an archaeon: cloning, sequencing, and expression of a gene encoding isocitrate dehydrogenase from a hyperthermophilic archaebacterium, Caldococcus noboribetus

Aoshima, M.; Yamagishi, A.; Oshima, T.; Arch. Biochem. Biophys. 336, 77-85 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expression of the gene in Escherichia coli by connecting it with the T7 promoter
Caldococcus noboribetus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
48000
-
x * 48000, SDS-PAGE
Caldococcus noboribetus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Caldococcus noboribetus
strict substrate and coenzyme specificity
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Caldococcus noboribetus
P96318
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
strict substrate and coenzyme specificity
726802
Caldococcus noboribetus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
?
x * 48000, SDS-PAGE
Caldococcus noboribetus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
80
-
assay at
Caldococcus noboribetus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.42
70
80
stable
Caldococcus noboribetus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7
-
assay at
Caldococcus noboribetus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expression of the gene in Escherichia coli by connecting it with the T7 promoter
Caldococcus noboribetus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
48000
-
x * 48000, SDS-PAGE
Caldococcus noboribetus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Caldococcus noboribetus
strict substrate and coenzyme specificity
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
strict substrate and coenzyme specificity
726802
Caldococcus noboribetus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
?
x * 48000, SDS-PAGE
Caldococcus noboribetus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
80
-
assay at
Caldococcus noboribetus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.42
70
80
stable
Caldococcus noboribetus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7
-
assay at
Caldococcus noboribetus