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Literature summary extracted from

  • Jia, M.; Mu, W.; Chu, F.; Zhang, X.; Jiang, B.; Zhou, L.L.; Zhang, T.
    A D-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for D-psicose production: cloning, expression, purification, and characterization (2013), Appl. Microbiol. Biotechnol., 98, 717-725.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.30 synthesis for the high production of D-psicose from D-fructose, the reaction reaches equilibrium after 160 min with the highest turnover yield (28.8%) observed at 55 °C Enterocloster bolteae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.30 expressed with a C-terminal 6His-tag in Escherichia coli Enterocloster bolteae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.30 EDTA
-
 Enterocloster bolteae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.30 27.4
-
 D-psicose pH 7.0, 55°C Enterocloster bolteae
5.1.3.30 59.8
-
 D-fructose pH 7.0, 55°C Enterocloster bolteae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.30 Co2+ metalloprotein that exhibits the highest activity in the presence of Co2+ Enterocloster bolteae
5.1.3.30 Mn2+ enhances activity Enterocloster bolteae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.30 33015
-
 4 * 33015, calculated from sequence Enterocloster bolteae
5.1.3.30 34000
-
 4 * 34000, SDS-PAGE Enterocloster bolteae
5.1.3.30 139000
-
 gel filtration Enterocloster bolteae

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.30 Enterocloster bolteae A8RG82
-
-
5.1.3.30 Enterocloster bolteae ATCC BAA-613 A8RG82
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.30
-
 Enterocloster bolteae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.30 D-fructose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae D-psicose
-
 r
5.1.3.30 D-fructose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-psicose
-
 r
5.1.3.30 D-psicose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae D-fructose
-
 r
5.1.3.30 D-psicose under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31, relative activity with D-fructose is 66.4% of the activity with D-psicose Enterocloster bolteae ATCC BAA-613 D-fructose
-
 r
5.1.3.30 D-tagatose
-
 Enterocloster bolteae D-sorbose
-
 r
5.1.3.30 D-tagatose
-
 Enterocloster bolteae ATCC BAA-613 D-sorbose
-
 r
5.1.3.30 additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate. Catalytic efficiency (kcat/KM) of D-tagatose is 127fold lower than catalytic efficiency of D-psicose Enterocloster bolteae ?
-
 ?
5.1.3.30 additional information no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate. Catalytic efficiency (kcat/KM) of D-tagatose is 127fold lower than catalytic efficiency of D-psicose Enterocloster bolteae ATCC BAA-613 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.30 tetramer 4 * 34000, SDS-PAGE Enterocloster bolteae
5.1.3.30 tetramer 4 * 33015, calculated from sequence Enterocloster bolteae

Synonyms

EC Number Synonyms Comment Organism
5.1.3.30 CLOBOL_00069 locus name Enterocloster bolteae
5.1.3.30 DPEase ambiguous Enterocloster bolteae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.30 55
-
-
 Enterocloster bolteae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.30 40 70 40°C about 50% of maximal activity, 70°C: about 40% of maximal activity Enterocloster bolteae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.30 45
-
 in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended Enterocloster bolteae
5.1.3.30 50
-
 in the absence of Co2+, the activity is very stable below 45 °C but decreases at temperatures above 50 °C with increasing reaction times. When incubated with 0.4 mM Co2+, the half-lives (t1/2) of the enzyme are extended Enterocloster bolteae
5.1.3.30 55
-
 half-life: 156 min Enterocloster bolteae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.30 49
-
 D-psicose pH 7.0, 55°C Enterocloster bolteae
5.1.3.30 59
-
 D-fructose pH 7.0, 55°C Enterocloster bolteae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.30 7
-
-
 Enterocloster bolteae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.30 5.5 8.5 pH 5.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Enterocloster bolteae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.30 6 7.5 after incubation at 4 °C for 2 h, more than 90% of the residual activity is retained at pH values ranging from of 6.0–7.5 Enterocloster bolteae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.30 Enterocloster bolteae calculated from sequence
-
 5.02

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.30 0.99
-
 D-fructose pH 7.0, 55°C Enterocloster bolteae
5.1.3.30 1.78
-
 D-psicose pH 7.0, 55°C Enterocloster bolteae