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Literature summary extracted from

  • Koga, Y.; Tanaka, S.; Sakudo, A.; Tobiume, M.; Aranishi, M.; Hirata, A.; Takano, K.; Ikuta, K.; Kanaya, S.
    Proteolysis of abnormal prion protein with a thermostable protease from Thermococcus kodakarensis KOD1 (2013), Appl. Microbiol. Biotechnol., 98, 2113-2120.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.B57 medicine the abnormal prion protein (scrapie-associated prion protein, PrP(Sc)) is considered to be included in the group of infectious agents of transmissible spongiform encephalopathies. Since PrP(Sc) is highly resistant to normal sterilization procedures, the decontamination of PrP(Sc) is a significant public health issue. The hyperthermostable protease, Tk-subtilisin, can be an effective agent for medical decontamination of of PrP(Sc). Rather small amounts of Tk-subtilisin (0.3 U) are required to degrade PrP(Sc) at 100 °C and pH 8.0. In addition, Tk-subtilisin is observed to degrade PrP(Sc) in the presence of sodium dodecyl sulfate or other industrial surfactants. Basic information for the practical use of the proteolytic enzyme for PrP(Sc) degradation is provided Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B57 expression in Escherichia coli BL21(DE3) Thermococcus kodakarensis

General Stability

EC Number General Stability Organism
3.4.21.B57 the activity is retained or even enhanced in the presence of nonionic, cationic (except in the presence of SANIZOL C), and amphoteric surfactants at both 0.1 and 1% (w/v) Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B57 DFP
-
 Thermococcus kodakarensis
3.4.21.B57 additional information the activity is retained or even enhanced in the presence of nonionic, cationic (except in the presence of SANIZOL C), and amphoteric surfactants at both 0.1 and 1% (w/v) Thermococcus kodakarensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.B57 guanidine-HCl Tk-subtilisin retains its specific activity in 6 M guanidine HCl after 60 min incubation at room temperature Thermococcus kodakarensis
3.4.21.B57 SDS Tk-subtilisin shows no significant reduction of specific activity after 60 min incubation at room temperature with 5% SDS Thermococcus kodakarensis
3.4.21.B57 urea Tk-subtilisin retains its specific activity in 8 M urea after 60 min incubation at room temperature Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B57 Thermococcus kodakarensis P58502 sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B57 abnormal prion protein PrP(Sc) + H2O the abnormal prion protein (scrapie-associated prion protein, PrP(Sc)) is considered to be included in the group of infectious agents of transmissible spongiform encephalopathies. Although PrP(Sc) is known to be resistant toward proteolytic enzymes, Tk-subtilisin is able to degrade PrP(Sc) under extreme conditions Thermococcus kodakarensis ?
-
 ?
3.4.21.B57 N-succinyl-AAPF-4-nitroanilide + H2O
-
 Thermococcus kodakarensis N-succinyl-AAPF + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B57 80
-
 assay at Thermococcus kodakarensis