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Literature summary extracted from
- The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866 (2014), Appl. Microbiol. Biotechnol., 98, 1349-1356.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.1.96 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
| 1.17.9.1 | expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas putida |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.96 | 0.00357 | - |
4-hydroxybenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida |  |
| 1.2.1.96 | 0.00636 | - |
4-hydroxy-3-methylbenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 0.01311 | - |
4-hydroxybenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 0.02055 | - |
4-hydroxy-3-methylbenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.96 | 4-hydroxybenzaldehyde + NADP+ + H2O | Pseudomonas putida | - |
4-hydroxybenzoic acid + NADPH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxybenzaldehyde + NADP+ + H2O | Pseudomonas putida NCIMB 9866 | - |
4-hydroxybenzoic acid + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.96 | Pseudomonas putida | Q59702 | - |
- |
| 1.2.1.96 | Pseudomonas putida NCIMB 9866 | Q59702 | - |
- |
| 1.17.9.1 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.96 | Ni2+-NTA column chromatography | Pseudomonas putida |
| 1.17.9.1 | using Ni-NTA-chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.96 | 4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida | 4-hydroxy-3-methylbenzoic acid + NADH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida NCIMB 9866 | 4-hydroxy-3-methylbenzoic acid + NADH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida | 4-hydroxy-3-methylbenzoic acid + NADPH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida NCIMB 9866 | 4-hydroxy-3-methylbenzoic acid + NADPH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxybenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida | 4-hydroxybenzoic acid + NADH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxybenzaldehyde + NAD+ + H2O | low activity with NAD+ as cosubstrate | Pseudomonas putida NCIMB 9866 | 4-hydroxybenzoic acid + NADH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxybenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida | 4-hydroxybenzoic acid + NADPH + H+ | - |
? | |
| 1.2.1.96 | 4-hydroxybenzaldehyde + NADP+ + H2O | - |
Pseudomonas putida NCIMB 9866 | 4-hydroxybenzoic acid + NADPH + H+ | - |
? | |
| 1.17.9.1 | 2,4-xylenol + acceptor + H2O | - |
Pseudomonas putida | 3-methyl-4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
| 1.17.9.1 | 4-cresol + acceptor + H2O | - |
Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.1.96 | p-hydroxybenzaldehyde dehydrogenase | - |
Pseudomonas putida |
| 1.2.1.96 | PchA | - |
Pseudomonas putida |
| 1.2.1.96 | PHBDD | - |
Pseudomonas putida |
| 1.17.9.1 | p-cresol methylhydroxylase | - |
Pseudomonas putida |
| 1.17.9.1 | pchF | - |
Pseudomonas putida |
| 1.17.9.1 | PCMH | - |
Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.96 | 0.0017 | - |
4-hydroxybenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 0.0017 | - |
4-hydroxy-3-methylbenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 0.071 | - |
4-hydroxy-3-methylbenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 0.26 | - |
4-hydroxybenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.9.1 | 9 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.96 | NADP+ | dependent on | Pseudomonas putida | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.17.9.1 | malfunction | using a gene-knockout it is shown that PCMH-encoding gene pchF is necessary for the catabolism of 2,4-xylenol | Pseudomonas putida |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.96 | 0.083 | - |
4-hydroxy-3-methylbenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 0.13 | - |
4-hydroxybenzaldehyde | with NAD+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 11.16 | - |
4-hydroxy-3-methylbenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
| 1.2.1.96 | 72.83 | - |
4-hydroxybenzaldehyde | with NADP+ as cosubstrate, at pH 8.0 and 30°C | Pseudomonas putida | |
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