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Literature summary extracted from

  • Du, J.; Li, L.; Ding, X.; Hu, H.; Lu, Y.; Zhou, S.
    Isolation and characterization of a novel cyanophycin synthetase from a deep-sea sediment metagenomic library (2013), Appl. Microbiol. Biotechnol., 97, 8619-8628.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.29 additional information the enzyme requires a sulfhydryl component uncultured bacterium
6.3.2.30 additional information the enzyme requires a sulfhydryl component uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha uncultured bacterium
6.3.2.30 gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), phylogenetic tree, subcloning in Escherichia coli strain DH5alpha uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.29 cytoplasm cyanophycin is deposited in the cytoplasm asmembraneless granules uncultured bacterium 5737
-
6.3.2.30 cytoplasm cyanophycin is deposited in the cytoplasm as membraneless granules uncultured bacterium 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.29 K+ required uncultured bacterium
6.3.2.29 Mg2+ required uncultured bacterium
6.3.2.30 K+ required uncultured bacterium
6.3.2.30 Mg2+ required uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.29 98000
-
 x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
6.3.2.29 100000
-
 x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
6.3.2.30 98000
-
 x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
6.3.2.30 100000
-
 x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp uncultured bacterium
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information uncultured bacterium cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg uncultured bacterium
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 additional information uncultured bacterium cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 uncultured bacterium M9UYB0 gene cphA49 from deep-sea sediment metagenomic library
-
6.3.2.30 uncultured bacterium M9UYB0 gene cphA49 from deep-sea sediment metagenomic library
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.29 recombinant His-tagged CphA49 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration uncultured bacterium
6.3.2.30 recombinant His-tagged CphA49 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 uncultured bacterium ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers uncultured bacterium ?
-
 ?
6.3.2.29 additional information the recombinant CphA49 exhibits strict primer dependency and broad substrate specificities. L-Lys and L-Glu can substitute for L-Asp, but with very low catalytic activity. No activity with L-Arg and L-citrulline, L-Arg and L-ornithine, L-Asp and L-citrulline, and L-Asp and L-ornithine uncultured bacterium ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 uncultured bacterium ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 additional information cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers uncultured bacterium ?
-
 ?
6.3.2.30 additional information the recombinant CphA49 exhibits strict primer dependency and broad substrate specificities. L-Lys and L-Glu can substitute for L-Arg, but with very low catalytic activity. No activity with L-Arg and L-citrulline, L-Arg and L-ornithine, L-Asp and L-citrulline, and L-Asp and L-ornithine uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.29 ? x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
6.3.2.30 ? x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 CphA
-
 uncultured bacterium
6.3.2.29 CphA49
-
 uncultured bacterium
6.3.2.29 cyanophycin synthetase
-
 uncultured bacterium
6.3.2.30 CphA
-
 uncultured bacterium
6.3.2.30 CphA49
-
 uncultured bacterium
6.3.2.30 cyanophycin synthetase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.29 40
-
 recombinant enzyme uncultured bacterium
6.3.2.30 40
-
 recombinant enzyme uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.29 10 70 activity range, profile overview uncultured bacterium
6.3.2.30 10 70 activity range, profile overview uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.29 40
-
 purified recombinant enzyme, stable up to uncultured bacterium
6.3.2.29 50
-
 purified recombinant enzyme, 60 min, over 8% activity remaining uncultured bacterium
6.3.2.29 70
-
 purified recombinant enzyme, 15 min, inactivation uncultured bacterium
6.3.2.30 40
-
 purified recombinant enzyme, stable up to uncultured bacterium
6.3.2.30 50
-
 purified recombinant enzyme, 60 min, over 8% activity remaining uncultured bacterium
6.3.2.30 70
-
 purified recombinant enzyme, 15 min, inactivation uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.29 9
-
 recombinant enzyme uncultured bacterium
6.3.2.30 9
-
 recombinant enzyme uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.29 7 11 activity range, high activity at a pH range of pH 8.0 to pH 10.5, recombinant enzyme, profile overview uncultured bacterium
6.3.2.30 7 11 activity range, high activity at a pH range of pH 8.0 to pH 10.5, recombinant enzyme, profile overview uncultured bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.29 ATP
-
 uncultured bacterium
6.3.2.30 ATP
-
 uncultured bacterium

General Information

EC Number General Information Comment Organism
6.3.2.29 evolution cyanophycin synthetase CphA49 belongs to NOR5 clade of Gammaproteobacteria enzymes uncultured bacterium
6.3.2.30 evolution cyanophycin synthetase CphA49 belongs to NOR5 clade of Gammaproteobacteria enzymes uncultured bacterium