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Literature summary extracted from

  • Min, K.; Kim, S.; Yum, T.; Kim, Y.; Sang, B.I.; Um, Y.
    Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum (2013), Appl. Microbiol. Biotechnol., 97, 5627-5634.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.4 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) activates about 2fold at 5-10 mM Clostridium acetobutylicum
4.1.1.4 methyl viologen activates 6.7fold at 10 mM Clostridium acetobutylicum

Application

EC Number Application Comment Organism
4.1.1.4 synthesis acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid in an enzymatic system for synthesis of 2-butanone from levulinic acid Clostridium acetobutylicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.4 recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), best induction over 24 h at 20°C with 1 mM IPTG Clostridium acetobutylicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.4 Azure B slight inhibition at 2-10 mM Clostridium acetobutylicum
4.1.1.4 levulinic acid substrate inhibition is observed with levulinic acid concentration higher than 5 mM Clostridium acetobutylicum
4.1.1.4 methylene blue complete inhibition at 2 mM Clostridium acetobutylicum
4.1.1.4 Zn2+ inhibits at above 5 mM Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.4 acetoacetate + H+ Clostridium acetobutylicum
-
 acetone + CO2
-
 ?
4.1.1.4 additional information Clostridium acetobutylicum acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.4 Clostridium acetobutylicum P23670
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.4 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.4 acetoacetate + H+
-
 Clostridium acetobutylicum acetone + CO2
-
 ?
4.1.1.4 levulinic acid + H+
-
 Clostridium acetobutylicum 2-butanone + CO2
-
 ?
4.1.1.4 additional information acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid Clostridium acetobutylicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.4 AADC
-
 Clostridium acetobutylicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.4 30
-
-
 Clostridium acetobutylicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.4 20 45 over 30% of maximal activity at 20°C and 45°C Clostridium acetobutylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.4 5
-
 with levulinic acid Clostridium acetobutylicum
4.1.1.4 6
-
 with acetoacetate Clostridium acetobutylicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.4 3.5 8 activity range, profile overview Clostridium acetobutylicum