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Literature summary extracted from

  • Meussen, B.J.; Weusthuis, R.A.; Sanders, J.P.; Graaff, L.H.
    Production of cyanophycin in Rhizopus oryzae through the expression of a cyanophycin synthetase encoding gene (2012), Appl. Microbiol. Biotechnol., 93, 1167-1174.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 gene cphA6803, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method Synechocystis sp.
6.3.2.29 gene cphA7120, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method Anabaena sp.
6.3.2.30 gene cphA6803, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method Synechocystis sp.
6.3.2.30 gene cphA7120, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Anabaena sp.
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Synechocystis sp.
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Anabaena sp. PCC 7120
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Anabaena sp.
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Synechocystis sp.
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Anabaena sp. PCC 7120
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Anabaena sp. P58572 gene cphA7120
-
6.3.2.29 Anabaena sp. PCC 7120 P58572 gene cphA7120
-
6.3.2.29 Synechocystis sp. P73833
-
-
6.3.2.30 Anabaena sp. P58572 gene cphA7120
-
6.3.2.30 Anabaena sp. PCC 7120 P58572 gene cphA7120
-
6.3.2.30 Synechocystis sp. P73833 gene cphA6803
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Anabaena sp. ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Synechocystis sp. ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Anabaena sp. PCC 7120 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Anabaena sp. ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Synechocystis sp. ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Anabaena sp. PCC 7120 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 cyanophycin synthetase
-
 Anabaena sp.
6.3.2.29 cyanophycin synthetase
-
 Synechocystis sp.
6.3.2.30 cyanophycin synthetase
-
 Anabaena sp.
6.3.2.30 cyanophycin synthetase
-
 Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.29 7.5
-
 assay at Anabaena sp.
6.3.2.29 7.5
-
 assay at Synechocystis sp.
6.3.2.30 7.5
-
 assay at Anabaena sp.
6.3.2.30 7.5
-
 assay at Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.29 ATP
-
 Anabaena sp.
6.3.2.29 ATP
-
 Synechocystis sp.
6.3.2.30 ATP
-
 Anabaena sp.
6.3.2.30 ATP
-
 Synechocystis sp.