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Literature summary extracted from

  • Schallmey, A.; Den Besten, G.; Teune, I.; Kembaren, R.; Janssen, D.
    Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca (2011), Appl. Microbiol. Biotechnol., 89, 1475-1485.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 expressed in Escherichia coli C43(DE3) cells Thermobifida fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.1 48000
-
x * 48000, SDS-PAGE Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Thermobifida fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.1 Q Sepharose column chromatography and HisTrap HP column chromatography Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 1,4-dimethoxybenzene + [reduced NADPH-hemoprotein reductase] + O2
-
Thermobifida fusca ?
-
?
1.14.14.1 anisole + [reduced NADPH-hemoprotein reductase] + O2
-
Thermobifida fusca ?
-
?
1.14.14.1 benzyl methyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 50% conversion Thermobifida fusca benzyl methyl sulfoxide + benzyl methyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 78:22 ?
1.14.14.1 ethyl phenyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 70% conversion Thermobifida fusca ethyl phenyl sulfoxide + ethyl phenyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 52:18 ?
1.14.14.1 ethylbenzene + [reduced NADPH-hemoprotein reductase] + O2
-
Thermobifida fusca (R)-1-phenylethanol + 1-phenylethanone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 88:12 ?
1.14.14.1 indole + [reduced NADPH-hemoprotein reductase] + O2
-
Thermobifida fusca (2Z)-2-(3-oxo-1,3-dihydro-2H-indol-2-ylidene)-1,2-dihydro-3H-indol-3-one + (3Z)-3-(3-oxo-1,3-dihydro-2H-indol-2-ylidene)-1,3-dihydro-2H-indol-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.1 methyl 4-methylphenyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 41% conversion Thermobifida fusca methyl 4-methylphenyl sulfoxide + methyl 4-methylphenyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 95:5 ?
1.14.14.1 methyl phenyl sulfide + O2 + NADH 55% conversion Thermobifida fusca methyl phenyl sulfoxide + methyl phenyl sulfone + NAD+ + H2O product ratio of 77:23 ?
1.14.14.1 additional information no activity with 3-acetylindole Thermobifida fusca ?
-
?
1.14.14.1 N-acetylindole + [reduced NADPH-hemoprotein reductase] + O2
-
Thermobifida fusca 1-acetyl-1,2-dihydro-3H-indol-3-one + 1-acetyl-1,3-dihydro-2H-indol-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.1 propylbenzene + [reduced NADPH-hemoprotein reductase] + O2
-
Thermobifida fusca (R)-1-phenyl-1-propanol + (R)-1-phenyl-1-propanol + 1-phenylpropan-1-one + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 79:7:14 ?
1.14.14.1 styrene + O2 + reduced putidaredoxin
-
Thermobifida fusca (S)-styrene oxide + 2-phenylethanol + oxidized putidaredoxin + H2O product ratio of 81:19 ?
1.14.14.1 toluene + O2 + NADH
-
Thermobifida fusca ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.1 ? x * 48000, SDS-PAGE Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 CYP154H1
-
Thermobifida fusca
1.14.14.1 cytochrome P450 monooxygenase
-
Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.1 50 67 the enzyme is moderately thermostable with an apparent melting temperature of 67°C and exhibits still 90% of initial activity after incubation at 50°C Thermobifida fusca