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Literature summary extracted from

  • Lee, S.J.; Lee, S.J.; Lee, Y.J.; Kim, S.B.; Kim, S.K.; Lee, D.W.
    Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability (2012), Appl. Environ. Microbiol., 78, 8813-8816.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.4 M33R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius
5.3.1.4 Q202R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius
5.3.1.4 R200E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
5.3.1.4 R216E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
5.3.1.4 R31E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
5.3.1.4 R48E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
5.3.1.4 V50R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius
5.3.1.4 Y218R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Alicyclobacillus acidocaldarius
-
 L-ribulose
-
 ?
5.3.1.4 L-arabinose Alicyclobacillus sp.
-
 L-ribulose
-
 ?
5.3.1.4 L-arabinose Alicyclobacillus sp. TP7
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Alicyclobacillus acidocaldarius
-
-
-
5.3.1.4 Alicyclobacillus sp.
-
-
-
5.3.1.4 Alicyclobacillus sp. TP7
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 L-arabinose
-
 Alicyclobacillus acidocaldarius L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Alicyclobacillus sp. L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Alicyclobacillus sp. TP7 L-ribulose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 6 6.5
-
 Alicyclobacillus acidocaldarius
5.3.1.4 8.5
-
-
 Alicyclobacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.4 5 9 the wild type enzyme shows half-lifes of activity of 14, 90 and 64.4 h at pH 5.0, 7.0, and 9.0, respectively Alicyclobacillus acidocaldarius
5.3.1.4 5 9 the wild type enzyme shows half-lifes of activity of 6.9, 201 and 334 h at pH 5.0, 7.0, and 9.0, respectively Alicyclobacillus sp.