BRENDA - Enzyme Database

Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle

Han, Y.; Hawkins, A.S.; Adams, M.W.; Kelly, R.M.; Appl. Environ. Microbiol. 78, 6194-6202 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
5.1.99.1
expression in Escherichia coli
Metallosphaera sedula
5.1.99.1
gene Msed_0639, DNA and amino acid sequence determination, phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
Metallosphaera sedula
5.4.99.2
expression in Escherichia coli
Metallosphaera sedula
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.1.99.1
Co2+
activates
Metallosphaera sedula
5.1.99.1
Co2+
the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Metallosphaera sedula
5.1.99.1
Mg2+
activates
Metallosphaera sedula
5.1.99.1
Mg2+
the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Metallosphaera sedula
5.1.99.1
Ni2+
activates
Metallosphaera sedula
5.1.99.1
Ni2+
the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Metallosphaera sedula
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.99.1
20000
-
2 * 20000, SDS-PAGE
Metallosphaera sedula
5.1.99.1
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Metallosphaera sedula
5.1.99.1
32100
-
gel filtration
Metallosphaera sedula
5.1.99.1
32100
-
recombinant His-tagged enzyme, gel filtration
Metallosphaera sedula
5.4.99.2
17000
-
2 * 17000 + 2 * 70000, SDS-PAGE
Metallosphaera sedula
5.4.99.2
70000
-
2 * 17000 + 2 * 70000, SDS-PAGE
Metallosphaera sedula
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula
-
(R)-methylmalonyl-CoA
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
(R)-methylmalonyl-CoA
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula DSM 5348
-
(R)-methylmalonyl-CoA
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula DSM 5348
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
(R)-methylmalonyl-CoA
-
-
?
5.4.99.2
(R)-methylmalonyl-CoA
Metallosphaera sedula
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
succinyl-CoA
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
Metallosphaera sedula DSM 5348
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
succinyl-CoA
-
-
r
Organism
EC Number
Organism
UniProt
Commentary
Textmining
5.1.99.1
Metallosphaera sedula
A4YEG2
-
-
5.1.99.1
Metallosphaera sedula DSM 5348
A4YEG2
-
-
5.4.99.2
Metallosphaera sedula
A4YEG1 and A4YIE3
A4YEG1: catalytic subunit of methylmalonyl-CoA mutase, A4YIE3: coenzyme B12-binding subunit of methylmalonyl-CoA mutase
-
5.4.99.2
Metallosphaera sedula DSM 5348
A4YEG1 and A4YIE3
A4YEG1: catalytic subunit of methylmalonyl-CoA mutase, A4YIE3: coenzyme B12-binding subunit of methylmalonyl-CoA mutase
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
5.1.99.1
-
Metallosphaera sedula
5.1.99.1
recombinant N-terminally His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography
Metallosphaera sedula
5.4.99.2
-
Metallosphaera sedula
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.1.99.1
additional information
transcriptomic analyses of Metallosphaera sedula grown under autotrophic versus heterotrophic conditions, overview. MCE expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions
Metallosphaera sedula
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1.99.1
218
-
75°C, pH not specified in the publication
Metallosphaera sedula
5.1.99.1
218
-
purified recombinant enzyme, pH 7.0, 75°C
Metallosphaera sedula
5.4.99.2
2.2
-
75°C, pH not specified in the publication
Metallosphaera sedula
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5.1.99.1
(S)-methylmalonyl-CoA
-
726729
Metallosphaera sedula
(R)-methylmalonyl-CoA
-
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula
(R)-methylmalonyl-CoA
-
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
-
726729
Metallosphaera sedula DSM 5348
(R)-methylmalonyl-CoA
-
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula DSM 5348
(R)-methylmalonyl-CoA
-
-
-
?
5.4.99.2
(R)-methylmalonyl-CoA
-
726729
Metallosphaera sedula
succinyl-CoA
-
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula
succinyl-CoA
-
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
-
726729
Metallosphaera sedula DSM 5348
succinyl-CoA
-
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula DSM 5348
succinyl-CoA
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.1.99.1
homodimer
2 * 20000, SDS-PAGE
Metallosphaera sedula
5.1.99.1
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Metallosphaera sedula
5.1.99.1
More
the putative three-dimensional structure of the enzyme is modeled using hypothetical protein BH1468, PDB ID 3OA4
Metallosphaera sedula
5.4.99.2
heterotetramer
2 * 17000 + 2 * 70000, SDS-PAGE
Metallosphaera sedula
Synonyms
EC Number
Synonyms
Commentary
Organism
5.1.99.1
MCE
-
Metallosphaera sedula
5.1.99.1
methylmalonyl-CoA epimerase
-
Metallosphaera sedula
5.1.99.1
methylmalonyl-coenzyme A epimerase
-
Metallosphaera sedula
5.1.99.1
Msed_0639
locus name
Metallosphaera sedula
5.4.99.2
MCM
-
Metallosphaera sedula
5.4.99.2
Msed_0638
locus name, catalytic subunit of methylmalonyl-CoA mutase
Metallosphaera sedula
5.4.99.2
Msed_2055
locus name, coenzyme B12-binding subunit of methylmalonyl-CoA mutase
Metallosphaera sedula
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.1.99.1
73
-
-
Metallosphaera sedula
5.1.99.1
75
-
assay at
Metallosphaera sedula
5.4.99.2
75
-
assay at
Metallosphaera sedula
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1.99.1
7
-
assay at
Metallosphaera sedula
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.99.1
expression in Escherichia coli
Metallosphaera sedula
5.1.99.1
gene Msed_0639, DNA and amino acid sequence determination, phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
Metallosphaera sedula
5.4.99.2
expression in Escherichia coli
Metallosphaera sedula
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.1.99.1
Co2+
activates
Metallosphaera sedula
5.1.99.1
Co2+
the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Metallosphaera sedula
5.1.99.1
Mg2+
activates
Metallosphaera sedula
5.1.99.1
Mg2+
the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Metallosphaera sedula
5.1.99.1
Ni2+
activates
Metallosphaera sedula
5.1.99.1
Ni2+
the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Metallosphaera sedula
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.99.1
20000
-
2 * 20000, SDS-PAGE
Metallosphaera sedula
5.1.99.1
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Metallosphaera sedula
5.1.99.1
32100
-
gel filtration
Metallosphaera sedula
5.1.99.1
32100
-
recombinant His-tagged enzyme, gel filtration
Metallosphaera sedula
5.4.99.2
17000
-
2 * 17000 + 2 * 70000, SDS-PAGE
Metallosphaera sedula
5.4.99.2
70000
-
2 * 17000 + 2 * 70000, SDS-PAGE
Metallosphaera sedula
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula
-
(R)-methylmalonyl-CoA
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
(R)-methylmalonyl-CoA
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula DSM 5348
-
(R)-methylmalonyl-CoA
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
Metallosphaera sedula DSM 5348
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
(R)-methylmalonyl-CoA
-
-
?
5.4.99.2
(R)-methylmalonyl-CoA
Metallosphaera sedula
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
succinyl-CoA
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
Metallosphaera sedula DSM 5348
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
succinyl-CoA
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.99.1
-
Metallosphaera sedula
5.1.99.1
recombinant N-terminally His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography
Metallosphaera sedula
5.4.99.2
-
Metallosphaera sedula
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.1.99.1
additional information
transcriptomic analyses of Metallosphaera sedula grown under autotrophic versus heterotrophic conditions, overview. MCE expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions
Metallosphaera sedula
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1.99.1
218
-
75°C, pH not specified in the publication
Metallosphaera sedula
5.1.99.1
218
-
purified recombinant enzyme, pH 7.0, 75°C
Metallosphaera sedula
5.4.99.2
2.2
-
75°C, pH not specified in the publication
Metallosphaera sedula
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5.1.99.1
(S)-methylmalonyl-CoA
-
726729
Metallosphaera sedula
(R)-methylmalonyl-CoA
-
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula
(R)-methylmalonyl-CoA
-
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
-
726729
Metallosphaera sedula DSM 5348
(R)-methylmalonyl-CoA
-
-
-
?
5.1.99.1
(S)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula DSM 5348
(R)-methylmalonyl-CoA
-
-
-
?
5.4.99.2
(R)-methylmalonyl-CoA
-
726729
Metallosphaera sedula
succinyl-CoA
-
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula
succinyl-CoA
-
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
-
726729
Metallosphaera sedula DSM 5348
succinyl-CoA
-
-
-
r
5.4.99.2
(R)-methylmalonyl-CoA
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
726729
Metallosphaera sedula DSM 5348
succinyl-CoA
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.1.99.1
homodimer
2 * 20000, SDS-PAGE
Metallosphaera sedula
5.1.99.1
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Metallosphaera sedula
5.1.99.1
More
the putative three-dimensional structure of the enzyme is modeled using hypothetical protein BH1468, PDB ID 3OA4
Metallosphaera sedula
5.4.99.2
heterotetramer
2 * 17000 + 2 * 70000, SDS-PAGE
Metallosphaera sedula
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.1.99.1
73
-
-
Metallosphaera sedula
5.1.99.1
75
-
assay at
Metallosphaera sedula
5.4.99.2
75
-
assay at
Metallosphaera sedula
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.1.99.1
7
-
assay at
Metallosphaera sedula
Expression
EC Number
Organism
Commentary
Expression
5.1.99.1
Metallosphaera sedula
expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation
up
5.1.99.1
Metallosphaera sedula
the enzyme expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions
up
5.4.99.2
Metallosphaera sedula
expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation
up
General Information
EC Number
General Information
Commentary
Organism
5.1.99.1
physiological function
methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase are responsible for producing succinyl-CoA from (S)-methylmalonyl-CoA. Succinyl-CoA is an intermediate connecting the 3-hydroxypropionate/4-hydroxybutyrate cycle and general carbon metabolism. Methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase may also be involved in degradation of propionate and branched-chain amino acids (valine, isoleucine, methionine). The enzyme has a role in CO2 fixation
Metallosphaera sedula
5.1.99.1
physiological function
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Metallosphaera sedula
5.4.99.2
physiological function
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Metallosphaera sedula
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.1.99.1
physiological function
methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase are responsible for producing succinyl-CoA from (S)-methylmalonyl-CoA. Succinyl-CoA is an intermediate connecting the 3-hydroxypropionate/4-hydroxybutyrate cycle and general carbon metabolism. Methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase may also be involved in degradation of propionate and branched-chain amino acids (valine, isoleucine, methionine). The enzyme has a role in CO2 fixation
Metallosphaera sedula
5.1.99.1
physiological function
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Metallosphaera sedula
5.4.99.2
physiological function
the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Metallosphaera sedula
Expression (protein specific)
EC Number
Organism
Commentary
Expression
5.1.99.1
Metallosphaera sedula
expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation
up
5.1.99.1
Metallosphaera sedula
the enzyme expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions
up
5.4.99.2
Metallosphaera sedula
expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation
up