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Literature summary extracted from

  • Dudek, H.M.; de Gonzalo, G.; Pazmino, D.E.; Stepniak, P.; Wyrwicz, L.S.; Rychlewski, L.; Fraaije, M.W.
    Mapping the substrate binding site of phenylacetone monooxygenase from Thermobifida fusca by mutational analysis (2011), Appl. Environ. Microbiol., 77, 5730-5738.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.92 A435Y the mutant is active only with bicyclo[3.2.0]hept-2-en-6-one Thermobifida fusca
1.14.13.92 A435Y the mutant shows less than 3% of wild type activity Thermobifida fusca
1.14.13.92 A442G the mutant shows 75% of wild type activity Thermobifida fusca
1.14.13.92 C65V the mutant shows 94% of wild type activity Thermobifida fusca
1.14.13.92 C65V/I67T the mutant shows 47% of wild type activity Thermobifida fusca
1.14.13.92 C65V/I67T/Q152F/S441A/A442G the mutant shows 31% of wild type activity Thermobifida fusca
1.14.13.92 C65V/I67T/Q93W the mutant shows 54% of wild type activity Thermobifida fusca
1.14.13.92 I339S the mutant shows 81% of wild type activity Thermobifida fusca
1.14.13.92 I67T the mutant shows 16% of wild type activity Thermobifida fusca
1.14.13.92 I67T/L338P the mutant shows less than 3% of wild type activity Thermobifida fusca
1.14.13.92 I67T/L338P/A435Y/A442G the mutant shows less than 3% of wild type activity Thermobifida fusca
1.14.13.92 I67T/L338P/A435Y/A442G/L443F/S444C the mutant shows less than 3% of wild type activity Thermobifida fusca
1.14.13.92 L153G inactive Thermobifida fusca
1.14.13.92 L153G the mutant shows less than 3% of wild type activity Thermobifida fusca
1.14.13.92 L338P the mutant shows 59% of wild type activity Thermobifida fusca
1.14.13.92 L443F the mutant shows 65% of wild type activity Thermobifida fusca
1.14.13.92 L447P the mutant shows 85% of wild type activity Thermobifida fusca
1.14.13.92 M446G the mutant shows 49% of wild type activity and is able to convert 1-indanone to 1-isochromanone Thermobifida fusca
1.14.13.92 Q152F the mutant shows 35% of wild type activity Thermobifida fusca
1.14.13.92 Q152F/A442G the mutant shows 37% of wild type activity Thermobifida fusca
1.14.13.92 Q152F/S441A/A442G the mutant shows 33% of wild type activity Thermobifida fusca
1.14.13.92 Q93W the mutant shows 65% of wild type activity Thermobifida fusca
1.14.13.92 Q93W/A442G/S444C/M446G/L447P the mutant shows 38% of wild type activity Thermobifida fusca
1.14.13.92 Q93W/S441A/A442G/S444C/M446G/L447P the mutant shows 40% of wild type activity Thermobifida fusca
1.14.13.92 S441A the mutant shows 73% of wild type activity Thermobifida fusca
1.14.13.92 S441A/A442G the mutant shows 56% of wild type activity Thermobifida fusca
1.14.13.92 S441A/A442G/S444C/M446G/L447P the mutant shows 41% of wild type activity Thermobifida fusca
1.14.13.92 S444C the mutant shows 66% of wild type activity Thermobifida fusca
1.14.13.92 V54I the mutant shows 65% of wild type activity Thermobifida fusca
1.14.13.92 V54I/C65V/I67T/Q93W/I339S/S441A/A442G/S444C/M446G/L447P the mutant shows 5% of wild type activity Thermobifida fusca
1.14.13.92 V54I/C65V/I67T/Q93W/Q152F/I339S/S441A/A442G/S444C/M446G/L447P the mutant shows less than 3% of wild type activity Thermobifida fusca
1.14.13.92 V54I/C65V/I67T/Q93W/Q152F/L153G/I339S/S441A/A442G/S444C/M446G/L447P the mutant shows less than 3% of wild type activity Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.92 phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
 benzyl acetate + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 thioanisole + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 PAMO
-
 Thermobifida fusca

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 FAD
-
 Thermobifida fusca
1.14.13.92 NADPH
-
 Thermobifida fusca