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Literature summary extracted from

  • Pulido, M.; Saito, K.; Tanaka, S.; Koga, Y.; Morikawa, M.; Takano, K.; Kanaya, S.
    Ca2+-dependent maturation of subtilisin from a hyperthermophilic archaeon, Thermococcus kodakaraensis: the propeptide is a potent inhibitor of the mature domain but is not required for its folding (2006), Appl. Environ. Microbiol., 72, 4154-4162.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B57 the propeptide and mature domain are independently overproduced in Escherichia coli Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B57 Thermococcus kodakaraensis subtilisin propeptide potent noncompetitive inhibitor of the mature domain Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B57 4
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 20°C, pH 9.5 Thermococcus kodakarensis
3.4.21.B57 8
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 80°C, pH 9.5, the Km value of 8.0 mM represents the apparent value, because the solubility of the substrate at pH 9.5 is too low to increase its concentration beyond 5 mM Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B57 Thermococcus kodakarensis P58502 sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106)
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B57 proteolytic modification pro-subtilisin is inactive in the absence of Ca2+ but is activated upon autoprocessing and degradation of propeptide in the presence of Ca2+ at 80°C. This maturation process is completed within 30 min at 80°C but is bound at an intermediate stage, in which the propeptide is autoprocessed from the mature domain (mat-subtilisin) but forms an inactive complex with mat-subtilisin*, at lower temperatures. At 80°C, approximately 30% of the pro-subtilisin is autoprocessed into propeptide and mat-subtilisin, and the other 70% is completely degraded to small fragments. mat-Subtilisin is inactive in the absence of Ca2+ but is activated upon incubation with Ca2+ at 80°C Thermococcus kodakarensis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B57
-
 Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.B57 13
-
 20°C, pH 9.5 Thermococcus kodakarensis
3.4.21.B57 140
-
 80°C, pH 9.5 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B57 azocasein + H2O
-
 Thermococcus kodakarensis ?
-
 ?
3.4.21.B57 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermococcus kodakarensis N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B57 90
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.B57 80
-
 stable for at least 3 h Thermococcus kodakarensis
3.4.21.B57 90
-
 the enzyme loses half of its activity in 9 h Thermococcus kodakarensis
3.4.21.B57 100
-
 the enzyme loses half of its activity in 50 min Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B57 26
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 20°C, pH 9.5 Thermococcus kodakarensis
3.4.21.B57 290
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 80°C, pH 9.5 Thermococcus kodakarensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.B57 0.000025
-
 Thermococcus kodakaraensis subtilisin propeptide 20°C, pH 9.5 Thermococcus kodakarensis