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Literature summary extracted from

  • Kannan, Y.; Koga, Y.; Inoue, Y.; Haruki, M.; Takagi, M.; Imanaka, T.; Morikawa, M.; Kanaya, S.
    Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence (2001), Appl. Environ. Microbiol., 67, 2445-2452.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B57 overproduced in Escherichia coli in a form with a putative prosequence in inclusion bodies, solubilized in the presence of 8 M urea, and refolded and converted to an active molecule. The enzyme is refolded in a form with a putative prosequence Thermococcus kodakarensis

General Stability

EC Number General Stability Organism
3.4.21.B57 the enzyme is stabilized in the presence of the Ca2+ Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.B57 CaCl2 highest activity in the presence of 5 mM CaCl2. The enzyme exhibits 70% and 80% of the maximal activity in the presence of 1 and 100 mM CaCl2, respectively Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B57 40000
-
 gel filtration Thermococcus kodakarensis
3.4.21.B57 43783
-
 1 * 43783, calculation from sequence Thermococcus kodakarensis
3.4.21.B57 44000
-
 1 * 44000, SDS-PAGE Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B57 Thermococcus kodakarensis P58502 sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B57
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B57 additional information broad substrate specificity, with a slight preference for aromatic or large nonpolar P1 substrate residues Thermococcus kodakarensis ?
-
 ?
3.4.21.B57 N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O 24.3% compared to the activity with N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide Thermococcus kodakarensis N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
3.4.21.B57 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermococcus kodakarensis N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B57 monomer 1 * 44000, SDS-PAGE Thermococcus kodakarensis
3.4.21.B57 monomer 1 * 43783, calculation from sequence Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B57 80
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.B57 40 90 the enzyme exhibits 10% to 20% of the maximal activity at 40°C or 90°C Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.B57 80
-
 half life: more than 60 min, in the presence of 50 mM CaCl2 Thermococcus kodakarensis
3.4.21.B57 90
-
 half life: 20 min, in the presence of 50 mM CaCl2 Thermococcus kodakarensis
3.4.21.B57 100
-
 half life: 7 min, in the presence of 50 mM CaCl2 Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B57 9.5
-
-
 Thermococcus kodakarensis