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Literature summary extracted from

  • Hei, D.J.; Clark, D.S.
    Pressure stabilization of proteins from extreme thermophiles (1994), Appl. Environ. Microbiol., 60, 932-939.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.12.99.6 pressure of 50.7 MPa destabilizes the enzyme Methanothermococcus thermolithotrophicus
1.12.99.6 pressure of 50.7 MPa destabilizes the enzyme Methanococcus maripaludis
1.12.99.6 pressure of 50.7 MPa has a substantial stabilizing effect on the enzyme Methanocaldococcus jannaschii
1.12.99.6 pressure of 50.7 MPa has a substantial stabilizing effect on the enzyme Methanotorris igneus
1.12.99.6 the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 28 min Methanococcus maripaludis
1.12.99.6 the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 30 min Methanocaldococcus jannaschii
1.12.99.6 the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 49 min Methanothermococcus thermolithotrophicus
1.12.99.6 the half-life for inactivation of the hydrogenase at pressure of 1.0 MPa is 96 min Methanotorris igneus

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Methanocaldococcus jannaschii
-
-
-
1.12.99.6 Methanococcus maripaludis
-
-
-
1.12.99.6 Methanothermococcus thermolithotrophicus
-
-
-
1.12.99.6 Methanotorris igneus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 partial Methanothermococcus thermolithotrophicus
1.12.99.6 partial Methanocaldococcus jannaschii
1.12.99.6 partial Methanococcus maripaludis
1.12.99.6 partial Methanotorris igneus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + oxidized methyl viologen
-
Methanothermococcus thermolithotrophicus H+ + reduced methyl viologen
-
?
1.12.99.6 H2 + oxidized methyl viologen
-
Methanocaldococcus jannaschii H+ + reduced methyl viologen
-
?
1.12.99.6 H2 + oxidized methyl viologen
-
Methanococcus maripaludis H+ + reduced methyl viologen
-
?
1.12.99.6 H2 + oxidized methyl viologen
-
Methanotorris igneus H+ + reduced methyl viologen
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.99.6 90
-
an increase in pressure from 1.0 to 50.7 MPa increases the half-life of the hydrogenase at 90°C by about 400% Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 7.5
-
assay at Methanothermococcus thermolithotrophicus
1.12.99.6 7.5
-
assay at Methanocaldococcus jannaschii
1.12.99.6 7.5
-
assay at Methanococcus maripaludis
1.12.99.6 7.5
-
assay at Methanotorris igneus