EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.99.2 | synthesis | tyrosine phenol lyase is an important enzyme for the synthesis of pharmaceutical aromatic amino acids, viz. L-tyrosine and L-DOPA | Citrobacter freundii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.99.2 | 0.446 | - |
L-tyrosine | pH 8.5, 30°C | Citrobacter freundii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.99.2 | intracellular | - |
Citrobacter freundii | 5622 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 52000 | - |
4 * 52000, SDS-PAGE | Citrobacter freundii |
4.1.99.2 | 220000 | - |
native enzyme, native PAGE | Citrobacter freundii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.2 | L-3-4 dihydroxyphenylalanine + H2O | Citrobacter freundii | L-DOPA, alpha-beta elimination | catechol + pyruvate + NH3 | - |
r | |
4.1.99.2 | L-tyrosine + H2O | Citrobacter freundii | alpha-beta elimination | phenol + pyruvate + NH3 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.2 | Citrobacter freundii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.2 | native enzyme 35fold to homogeneity by ammonium sulfate fractionation, dialysis, and hydrophobic interaction chromatography | Citrobacter freundii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 1.1 | - |
purified enzyme, pH 8.5, 30°C | Citrobacter freundii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.2 | L-3-4 dihydroxyphenylalanine + H2O | L-DOPA, alpha-beta elimination | Citrobacter freundii | catechol + pyruvate + NH3 | - |
r | |
4.1.99.2 | L-asparagine + H2O | - |
Citrobacter freundii | ? | - |
? | |
4.1.99.2 | L-methionine + H2O | - |
Citrobacter freundii | ? | - |
? | |
4.1.99.2 | L-phenylalanine + H2O | low activity | Citrobacter freundii | ? | - |
? | |
4.1.99.2 | L-tyrosine + H2O | alpha-beta elimination | Citrobacter freundii | phenol + pyruvate + NH3 | - |
r | |
4.1.99.2 | L-valine + H2O | - |
Citrobacter freundii | ? | - |
? | |
4.1.99.2 | additional information | alanine, arginine, aspartate, cysteine, glutamine, glutamate, glycine, histidine, isoleucine, leucine, lysine, proline, serine, threonine, tryptophan are poor or no substrates, substrate specificity of purified tyrosine phenol lyase, overview | Citrobacter freundii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.99.2 | More | N-terminal sequences comparisons, overview | Citrobacter freundii |
4.1.99.2 | tetramer | 4 * 52000, SDS-PAGE | Citrobacter freundii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.2 | tyrosine phenol lyase | - |
Citrobacter freundii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 45 | - |
- |
Citrobacter freundii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 30 | 55 | activity range, profile, overview | Citrobacter freundii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 35 | - |
purified native enzyme, half-life is 130 min | Citrobacter freundii |
4.1.99.2 | 45 | - |
purified native enzyme, half-life is 52 min | Citrobacter freundii |
4.1.99.2 | 55 | - |
purified native enzyme, half-life is 10 min | Citrobacter freundii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 8.5 | - |
- |
Citrobacter freundii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.2 | pyridoxal 5'-phosphate | dependent on, highest activity at 0.015-0.02 mM | Citrobacter freundii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.2 | metabolism | tyrosine phenol lyase catalyses the biosynthesis of L-tyrosine/L-DOPA from ammonium, pyruvate and phenolic substrates through the reverse of alpha- and beta-elimination reaction | Citrobacter freundii |