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Literature summary extracted from

  • Salonen, N.; Nyyssoelae, A.; Salonen, K.; Turunen, O.
    Bifidobacterium longum L-arabinose isomerase - overexpression in Lactococcus lactis, purification, and characterization (2012), Appl. Biochem. Biotechnol., 168, 392-405.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expressed in Lactococcus lactis Bifidobacterium longum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 Co2+ moderate inhibitory effect at 1 mM Bifidobacterium longum
5.3.1.4 EDTA 30% inhibition at 1 mM Bifidobacterium longum
5.3.1.4 Fe2+ strong inhibitory effect, over 70% of the activity is lost already at 0.5 mM Fe2+ Bifidobacterium longum
5.3.1.4 Fe3+ strong inhibitory effect, over 70% of the activity is lost already at 0.5 mM Fe3+ Bifidobacterium longum
5.3.1.4 Mn2+ moderate inhibitory effect at 1 mM Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 120
-
L-arabinose at pH 6.5 and 45°C Bifidobacterium longum
5.3.1.4 590
-
D-galactose at pH 6.5 and 45°C Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Ca2+ the addition of Ca2+ has slightly positive but no significant effect on the enzyme activity Bifidobacterium longum
5.3.1.4 Mg2+ the addition of Mg2+ has slightly positive but no significant effect on the enzyme activity Bifidobacterium longum
5.3.1.4 additional information the enzyme has very low requirement for metal ions for catalytic activity, but it is stabilized by divalent metal ions (Mg2+, Mn2+) Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 55000
-
x * 55000, SDS-PAGE Bifidobacterium longum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Bifidobacterium longum
-
L-ribulose 11% yield after 2 h of incubation at 35°C ?
5.3.1.4 L-arabinose Bifidobacterium longum NRRL B-41409
-
L-ribulose 11% yield after 2 h of incubation at 35°C ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Bifidobacterium longum I3ZR32
-
-
5.3.1.4 Bifidobacterium longum NRRL B-41409 I3ZR32
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 DEAE Sepharose column chromatography and hydrophobic interaction chromatography Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
Bifidobacterium longum D-tagatose 36% yield after 6 days of incubation at 35°C ?
5.3.1.4 D-galactose
-
Bifidobacterium longum NRRL B-41409 D-tagatose 36% yield after 6 days of incubation at 35°C ?
5.3.1.4 L-arabinose
-
Bifidobacterium longum L-ribulose 11% yield after 2 h of incubation at 35°C ?
5.3.1.4 L-arabinose
-
Bifidobacterium longum NRRL B-41409 L-ribulose 11% yield after 2 h of incubation at 35°C ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 55000, SDS-PAGE Bifidobacterium longum

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 L-AI
-
Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 55
-
-
Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 25 55 the enzyme maintains its full activity for 2 days at 25 and 35°C, and over 50% of its full activity for 2 days at 45°C (half-life 52 h). At 55°C, the activity decreases rapidly (half-life 3 h) Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 6 6.5
-
Bifidobacterium longum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.4 4 8 the enzyme retains over 70% of ist relative activity for 5 days between pH 6.0 and 7.0. At pH 5.0 and 8.0, the relative activity decreases relatively rapidly to less than 40% of the initial activity (half-lives 6 and 28 h at pH 5.0 and 8.0, respectively), after which the decrease rate levels off. At pH 4.0, all activity is rapidly lost (half-life less than 1 h) Bifidobacterium longum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.4 0.012
-
D-galactose at pH 6.5 and 45°C Bifidobacterium longum
5.3.1.4 0.8
-
L-arabinose at pH 6.5 and 45°C Bifidobacterium longum