EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.4 | expressed in Lactococcus lactis | Bifidobacterium longum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.4 | Co2+ | moderate inhibitory effect at 1 mM | Bifidobacterium longum | |
5.3.1.4 | EDTA | 30% inhibition at 1 mM | Bifidobacterium longum | |
5.3.1.4 | Fe2+ | strong inhibitory effect, over 70% of the activity is lost already at 0.5 mM Fe2+ | Bifidobacterium longum | |
5.3.1.4 | Fe3+ | strong inhibitory effect, over 70% of the activity is lost already at 0.5 mM Fe3+ | Bifidobacterium longum | |
5.3.1.4 | Mn2+ | moderate inhibitory effect at 1 mM | Bifidobacterium longum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.4 | 120 | - |
L-arabinose | at pH 6.5 and 45°C | Bifidobacterium longum | |
5.3.1.4 | 590 | - |
D-galactose | at pH 6.5 and 45°C | Bifidobacterium longum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.4 | Ca2+ | the addition of Ca2+ has slightly positive but no significant effect on the enzyme activity | Bifidobacterium longum | |
5.3.1.4 | Mg2+ | the addition of Mg2+ has slightly positive but no significant effect on the enzyme activity | Bifidobacterium longum | |
5.3.1.4 | additional information | the enzyme has very low requirement for metal ions for catalytic activity, but it is stabilized by divalent metal ions (Mg2+, Mn2+) | Bifidobacterium longum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 55000 | - |
x * 55000, SDS-PAGE | Bifidobacterium longum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.4 | L-arabinose | Bifidobacterium longum | - |
L-ribulose | 11% yield after 2 h of incubation at 35°C | ? | |
5.3.1.4 | L-arabinose | Bifidobacterium longum NRRL B-41409 | - |
L-ribulose | 11% yield after 2 h of incubation at 35°C | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.4 | Bifidobacterium longum | I3ZR32 | - |
- |
5.3.1.4 | Bifidobacterium longum NRRL B-41409 | I3ZR32 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.4 | DEAE Sepharose column chromatography and hydrophobic interaction chromatography | Bifidobacterium longum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.4 | D-galactose | - |
Bifidobacterium longum | D-tagatose | 36% yield after 6 days of incubation at 35°C | ? | |
5.3.1.4 | D-galactose | - |
Bifidobacterium longum NRRL B-41409 | D-tagatose | 36% yield after 6 days of incubation at 35°C | ? | |
5.3.1.4 | L-arabinose | - |
Bifidobacterium longum | L-ribulose | 11% yield after 2 h of incubation at 35°C | ? | |
5.3.1.4 | L-arabinose | - |
Bifidobacterium longum NRRL B-41409 | L-ribulose | 11% yield after 2 h of incubation at 35°C | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.4 | ? | x * 55000, SDS-PAGE | Bifidobacterium longum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.4 | L-AI | - |
Bifidobacterium longum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 55 | - |
- |
Bifidobacterium longum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 25 | 55 | the enzyme maintains its full activity for 2 days at 25 and 35°C, and over 50% of its full activity for 2 days at 45°C (half-life 52 h). At 55°C, the activity decreases rapidly (half-life 3 h) | Bifidobacterium longum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 6 | 6.5 | - |
Bifidobacterium longum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 4 | 8 | the enzyme retains over 70% of ist relative activity for 5 days between pH 6.0 and 7.0. At pH 5.0 and 8.0, the relative activity decreases relatively rapidly to less than 40% of the initial activity (half-lives 6 and 28 h at pH 5.0 and 8.0, respectively), after which the decrease rate levels off. At pH 4.0, all activity is rapidly lost (half-life less than 1 h) | Bifidobacterium longum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.4 | 0.012 | - |
D-galactose | at pH 6.5 and 45°C | Bifidobacterium longum | |
5.3.1.4 | 0.8 | - |
L-arabinose | at pH 6.5 and 45°C | Bifidobacterium longum |