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Literature summary extracted from

  • Dilley, D.R.; Wang, Z.; Kadirjan-Kalbach, D.K.; Ververidis, F.; Beaudry, R.; Padmanabhan, K.
    1-Aminocyclopropane-1-carboxylic acid oxidase reaction mechanism and putative post-translational activities of the ACCO protein (2013), AoB plants, 5, plt031.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 2,4-pteridinediol i.e. lumazine, competitively activates the enzyme with respect to ascorbate Malus domestica
1.14.17.4 bicarbonate 20 mM bicarbonate pretreatment for 20 min is sufficient to protect and activate the enzyme Malus domestica
1.14.17.4 cyanide the enzyme is activated by cyanide concentrations between 0.1 and 1 mM with most efficient activation at 0.5 mM Malus domestica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 expressed in Escherichia coli BL21(DE3) cells Malus domestica

Protein Variants

EC Number Protein Variants Comment Organism
1.14.17.4 C133A the mutant showsincreased activity compared to the wild type enzyme Malus domestica
1.14.17.4 C133P the mutant shows slightly increased activity compared to the wild type enzyme Malus domestica
1.14.17.4 C165A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 C28A the mutant shows increased activity compared to the wild type enzyme Malus domestica
1.14.17.4 E294F the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 E297L the mutant shows strongly increased activity compared to the wild type enzyme Malus domestica
1.14.17.4 E301D the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 E301L the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 F187Y the mutant shows increased activity compared to the wild type enzyme Malus domestica
1.14.17.4 F300Q the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 F300Y the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K144E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158L the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158Q the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158Q/R175E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158Q/R175Q the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158R the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K158R/R175Q the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K172E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K199E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K230E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K230Q the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K230R the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K292E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K292R the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 K296E the mutant shows increased activity compared to the wild type enzyme Malus domestica
1.14.17.4 N216F the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 P298A the mutant shows strongly increased activity compared to the wild type enzyme Malus domestica
1.14.17.4 Q188A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 Q188K the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 Q188N the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175E the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175E/R244K the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175E/S246A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175E/T157A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175G the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175H the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175K the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R175Q the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R244K the mutant is less active than the native enzyme and has a 5fold higher Km value for 1-aminocyclopropane-1-carboxylate Malus domestica
1.14.17.4 R244K/S246A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R244K/S246A/T157A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R244K/T157A the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R299E inactive Malus domestica
1.14.17.4 R299H the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R299K the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 R299L the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 S246A the mutant is less active than the native enzyme and has a 3fold higher Km value for 1-aminocyclopropane-1-carboxylate Malus domestica
1.14.17.4 T157A the mutation does not affect the Km for 1-aminocyclopropane-1-carboxylate but drastically reduces enzyme activity Malus domestica
1.14.17.4 W203F the mutant shows reduced activity compared to the wild type enzyme Malus domestica
1.14.17.4 Y251F the mutant shows reduced activity compared to the wild type enzyme Malus domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 cyanide inhibitory beyond 1 mM Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.031
-
 1-aminocyclopropane-1-carboxylate mutant enzyme E301L, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.031
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R299L, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.036
-
 1-aminocyclopropane-1-carboxylate mutant enzyme F300Y, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.046
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158R, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.047
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R299K, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.051
-
 1-aminocyclopropane-1-carboxylate wild type enzyme, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.06
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.062
-
 1-aminocyclopropane-1-carboxylate mutant enzyme C28A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.062
-
 1-aminocyclopropane-1-carboxylate mutant enzyme Y251F, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.063
-
 1-aminocyclopropane-1-carboxylate mutant enzyme W203F, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.07
-
 1-aminocyclopropane-1-carboxylate mutant enzyme T157A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.078
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K199E, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.083
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175K, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.09
-
 1-aminocyclopropane-1-carboxylate mutant enzyme F187Y, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.094
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R299H, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.129
-
 1-aminocyclopropane-1-carboxylate mutant enzyme F300Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.139
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175H, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.142
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175G, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.158
-
 1-aminocyclopropane-1-carboxylate mutant enzyme N216F, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.173
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158E, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.175
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R244K, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.175
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R244K/S246A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.193
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.206
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158Q/R175Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.218
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.245
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R244K/S246A/T157A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.277
-
 1-aminocyclopropane-1-carboxylate mutant enzyme S246A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.279
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158R/R175Q, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.281
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158L, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.287
-
 1-aminocyclopropane-1-carboxylate mutant enzyme Q188N, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.379
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175E, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 0.659
-
 1-aminocyclopropane-1-carboxylate mutant enzyme K158Q/R175E, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 1.2
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175E/S246A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica
1.14.17.4 2.3
-
 1-aminocyclopropane-1-carboxylate mutant enzyme R175E/T157A, in 50 mM MOPS-HCl (pH 7.2), at 30°C Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Malus domestica
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Malus domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 ammonium sulfate precipitation, column chromatography, and gel filtration Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 additional information the enzyme becomes inactivated by fragmentation and apparently has intrinsic protease and transpeptidase activity Malus domestica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.4 1-aminocyclopropane-1-carboxylic acid oxidase
-
 Malus domestica
1.14.17.4 ACC oxidase
-
 Malus domestica
1.14.17.4 ACCO1
-
 Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.4 ascorbate
-
 Malus domestica

General Information

EC Number General Information Comment Organism
1.14.17.4 metabolism the enzyme catalyzes the final step in ethylene biosynthesis. The enzyme is involved in the ethylene signal transduction pathway not directly linked to the enzyme reaction Malus domestica