EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.9 | expressed in Escherichia coli | Pseudomonas fluorescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.19.9 | F103A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
1.14.19.9 | F103A/W455A | inactive | Pseudomonas fluorescens |
1.14.19.9 | H101A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
1.14.19.9 | W455A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.9 | 0.0499 | - |
tryptophan | His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.14.19.9 | 0.1986 | - |
tryptophan | His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.14.19.9 | 1.785 | - |
tryptophan | His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.14.19.9 | 1.814 | - |
tryptophan | His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.9 | tryptophan + FADH2 + Cl- + O2 + H+ | Pseudomonas fluorescens | - |
7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.9 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.19.9 | nickel chelating Sepharose column chromatography | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.9 | tryptophan + FADH2 + Cl- + O2 + H+ | - |
Pseudomonas fluorescens | 7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.9 | PrnA | - |
Pseudomonas fluorescens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.9 | 0.018 | - |
tryptophan | His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.14.19.9 | 0.021 | - |
tryptophan | His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.14.19.9 | 0.067 | - |
tryptophan | His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.14.19.9 | 0.11 | - |
tryptophan | His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.9 | flavin | dependent on | Pseudomonas fluorescens |