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Literature summary extracted from

  • Cao, T.P.; Choi, J.M.; Lee, S.J.; Lee, Y.J.; Lee, S.K.; Jun, Y.; Lee, D.W.; Lee, S.H.
    Crystallization and preliminary X-ray crystallographic analysis of L-arabinose isomerase from thermophilic Geobacillus kaustophilus (2014), Acta Crystallogr. Sect. F, 70, 108-112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expressed in Escherichia coli BL21(DE3) cells Geobacillus kaustophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.4 hanging drop vapor diffusion method, using 0.1 M imidazole hydrochloric acid pH 8.0, 1 M sodium citrate tribasic Geobacillus kaustophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Mn2+ required for activity Geobacillus kaustophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 56200
-
 6 * 56200, calculated from amino acid sequence Geobacillus kaustophilus
5.3.1.4 58000
-
 6 * 58000, His-tagged enzyme, SDS-PAGE Geobacillus kaustophilus
5.3.1.4 321000
-
 gel filtration Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Geobacillus kaustophilus
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Geobacillus kaustophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 Ni-NTA agarose column chromatography and Superdex 200 gel filtration Geobacillus kaustophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 0.84
-
 in the absence of Mn2+, at pH 7.5 and 60°C Geobacillus kaustophilus
5.3.1.4 4.03
-
 in the presence of Mn2+, at pH 7.5 and 60°C Geobacillus kaustophilus
5.3.1.4 4.57
-
 purified enzyme, at pH 7.5 and 60°C Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 L-arabinose
-
 Geobacillus kaustophilus L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 homohexamer 6 * 56200, calculated from amino acid sequence Geobacillus kaustophilus
5.3.1.4 homohexamer 6 * 58000, His-tagged enzyme, SDS-PAGE Geobacillus kaustophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.4 Geobacillus kaustophilus calculated from amino acid sequence
-
 5.4