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Literature summary extracted from

  • Miyazono, K.I.; Tsutsumi, K.; Ishino, Y.; Tanokura, M.
    Expression, high-pressure refolding, purification, crystallization and preliminary X-ray analysis of a novel single-strand-specific 3'-5' exonuclease PhoExo I from Pyrococcus horikoshii OT3 (2014), Acta Crystallogr. Sect. F, 70, 1076-1079.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.1 produced as inclusion bodies in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.11.1 solubilization of the inclusion bodies is performed by the high-pressure refolding method and highly purified protein is subjected to crystallization by the sitting-drop vapour-diffusion method at 20°C. A crystal of PhoExo I is obtained in a reservoir solution consisting of 0.1 M Tris-HCl pH 8.9, 27% PEG 6000 and diffracts X-rays to 1.52 A resolution. The crystal of PhoExo I belong to space group H32, with unit-cell parameters a = b = 112.07, c = 202.28 A. The crystal contains two PhoExo I molecules in the asymmetric unit Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.1 75000
-
 gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.1 Pyrococcus horikoshii
-
-
-
3.1.11.1 Pyrococcus horikoshii DSM 12428
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.1
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.1 5'-fluorescein-TTTTTTTTTTTTTTTTTTTTTTTTT-3' + H2O
-
 Pyrococcus horikoshii ?
-
 ?
3.1.11.1 5'-fluorescein-TTTTTTTTTTTTTTTTTTTTTTTTT-3' + H2O
-
 Pyrococcus horikoshii DSM 12428 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.1 homotrimer
-
 Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.1.11.1 PhoExo I
-
 Pyrococcus horikoshii
3.1.11.1 single-strand-specific 3'-5' exonuclease
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.11.1 65
-
 assay at Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.1 8
-
 assay at Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
3.1.11.1 physiological function the enzyme is involved in a Thermococcales-specific DNA-repair pathway Pyrococcus horikoshii