Literature summary extracted from
Penman, G.A.; Lockhart, D.E.; Ferenbach, A.; van Aalten, D.M.
Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus (2012), Acta Crystallogr. Sect. F, 68, 705-708.
Application
| EC Number |
Application |
Comment |
Organism |
|---|
| 5.4.99.9 |
drug development |
the enzyme is a potential drug target involved in the synthesis of the cell wall of this fungal pathogen |
Aspergillus fumigatus |
Cloned(Commentary)
| EC Number |
Cloned (Comment) |
Organism |
|---|
| 5.4.99.9 |
recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, and in strain B893 (DE3) pLysS for the selnomethionine-labeled enzyme |
Aspergillus fumigatus |
Crystallization (Commentary)
| EC Number |
Crystallization (Comment) |
Organism |
|---|
| 5.4.99.9 |
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 500 nl 15 mg/ml protein solution with 500 nl reservoir solution, containing 0.1 M HEPES, pH 7.5, 20% PEG 3350, 0.4 M ammonium sulfate, 4% formamide, and equilibration against 0.07 ml reservoir solution, for enzyme complex crystals, the enzyme is incubated for 10 min on ice with various substrate/inhibitor/reducing ligands 10 mM UDP-Galp, 10 mM UDP, 5 mM UDP-glucose, 5-20 mM sodium dithionite, respectively, before crystallization, 2 days, X-ray diffraction structure determination and analysis at 3.25 A resolution. Incorporation of selenomethionine is achieved, but the resulting crystals do not allow solution of the phase problem |
Aspergillus fumigatus |
Localization
| EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
|---|
Molecular Weight [Da]
| EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
|---|
| 5.4.99.9 |
57200 |
- |
x * 57200, about, sequence calculation |
Aspergillus fumigatus |
Organism
| EC Number |
Organism |
UniProt |
Comment |
Textmining |
|---|
| 5.4.99.9 |
Aspergillus fumigatus |
Q4W1X2 |
- |
- |
Purification (Commentary)
| EC Number |
Purification (Comment) |
Organism |
|---|
| 5.4.99.9 |
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS or B893 (DE3) pLysS by glutathione affinity chroatography and gel filtration |
Aspergillus fumigatus |
Subunits
| EC Number |
Subunits |
Comment |
Organism |
|---|
| 5.4.99.9 |
? |
x * 57200, about, sequence calculation |
Aspergillus fumigatus |
Synonyms
| EC Number |
Synonyms |
Comment |
Organism |
|---|
| 5.4.99.9 |
UGM |
- |
Aspergillus fumigatus |
Cofactor
| EC Number |
Cofactor |
Comment |
Organism |
Structure |
|---|
| 5.4.99.9 |
flavin |
flavoenzyme |
Aspergillus fumigatus |
|
General Information
| EC Number |
General Information |
Comment |
Organism |
|---|
| 5.4.99.9 |
physiological function |
the enzyme is involved in the synthesis of the cell wall |
Aspergillus fumigatus |
