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Literature summary extracted from

  • Yoshida, H.; Teraoka, M.; Yoshihara, A.; Izumori, K.; Kamitori, S.
    Overexpression, crystallization and preliminary X-ray diffraction analysis of L-ribose isomerase from Acinetobacter sp. strain DL-28 (2011), Acta Crystallogr. Sect. F, 67, 1281-1284.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.B3 expressed in Escherichia coli JM109 cells Acinetobacter sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.B3 hanging drop vapor diffusion method, using 0.2 M NaCl, 30% (w/v) PEG 400, 0.1 M HEPES pH 7.5 or 18-23% (w/v) PEG 400, 0.1 M HEPES pH 7.5, 0.16-0.2 M NaCl with or without 10-20 mM hexamminecobalt(III) chloride Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.B3 28700
-
 x * 28700, N-terminally His-tagged enzyme, SDS-PAGE Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.B3 L-ribulose Acinetobacter sp.
-
 L-ribose
-
 r
5.3.1.B3 L-ribulose Acinetobacter sp. DL-28
-
 L-ribose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.B3 Acinetobacter sp. Q93UQ5
-
-
5.3.1.B3 Acinetobacter sp. DL-28 Q93UQ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.B3 HisTrap affinity column chromatography, and gel filtration Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.B3 L-ribulose
-
 Acinetobacter sp. L-ribose
-
 r
5.3.1.B3 L-ribulose
-
 Acinetobacter sp. DL-28 L-ribose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.B3 ? x * 28700, N-terminally His-tagged enzyme, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
5.3.1.B3 L-RI
-
 Acinetobacter sp.