BRENDA - Enzyme Database

An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase

Davies, D.R.; Staker, B.L.; Abendroth, J.A.; Edwards, T.E.; Hartley, R.; Leonard, J.; Kim, H.; Rychel, A.L.; Hewitt, S.N.; Myler, P.J.; Stewart, L.J.; Acta Crystallogr. Sect. F 67, 1044-1050 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
dependent on
Burkholderia pseudomallei
Application
EC Number
Application
Commentary
Organism
5.4.2.11
drug development
as a key enzyme in glycolysis and energy metabolism, PGAM is a potential target for novel antibiotics
Burkholderia pseudomallei
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.11
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)-R3-pRARE2 cells
Burkholderia pseudomallei
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.11
purified recombinant His-tagged enzyme, in apoform or complexed with 2-phosphoserine, with vanadate + 3-phosphoglycerate, with vanadate + glycerol, with 2,3-bisphosphoglycerate + 3-phosphoglycerate, or with malonate, sitting-drop vapour diffusion by mixing 0.0004 ml reservoir solution with 0.0004 ml protein solution, containing 25 mM HEPES, pH 7.0, 500 mM NaCl, 5% v/v glycerol, 0.025% w/v sodium azide and 2 mM DTT, over a reservoir volume of 0.08 ml, the reservoir solution, containing 5% PEG 1000, 10% glycerol, 30% PEG 600, and 100 mM MES pH 7.5, is supplemented with the ligands at different concentrations, X-ray diffraction structure determination and analysis at 1.5-2.25 A resolution, molecular replacement and modeling
Burkholderia pseudomallei
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.11
vanadate
acting as a substrate mimic, enzyme binding structure, overview
Burkholderia pseudomallei
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Burkholderia pseudomallei
-
3-phospho-D-glycerate
-
-
r
5.4.2.11
2-phospho-D-glycerate
Burkholderia pseudomallei 1710b
-
3-phospho-D-glycerate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.11
Burkholderia pseudomallei
Q3JWH7
-
-
5.4.2.11
Burkholderia pseudomallei 1710b
Q3JWH7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.11
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-R3-pRARE2 cells by nickel affinity chromatography and gel filtration
Burkholderia pseudomallei
Reaction
EC Number
Reaction
Commentary
Organism
5.4.2.11
2-phospho-D-glycerate = 3-phospho-D-glycerate
mechanism of BPGM begins with an unphosphorylated enzyme, the active site histidine of which performs a nucleophilic SN2 attack on the 1,3-bisphosphoglycerate substrate to produce phosphohistidine and 3-phosphoglycerate. The 2'-hydroxyl group then performs a second nucleophilic attack that transfers the phosphate from the active site histidine to the substrate, forming 2,3-bisphosphoglycerate
Burkholderia pseudomallei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
726607
Burkholderia pseudomallei
3-phospho-D-glycerate
-
-
-
r
5.4.2.11
2-phospho-D-glycerate
-
726607
Burkholderia pseudomallei 1710b
3-phospho-D-glycerate
-
-
-
r
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.4.2.11
2,3-bisphosphoglycerate
dependent on
Burkholderia pseudomallei
Application (protein specific)
EC Number
Application
Commentary
Organism
5.4.2.11
drug development
as a key enzyme in glycolysis and energy metabolism, PGAM is a potential target for novel antibiotics
Burkholderia pseudomallei
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.11
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)-R3-pRARE2 cells
Burkholderia pseudomallei
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.11
purified recombinant His-tagged enzyme, in apoform or complexed with 2-phosphoserine, with vanadate + 3-phosphoglycerate, with vanadate + glycerol, with 2,3-bisphosphoglycerate + 3-phosphoglycerate, or with malonate, sitting-drop vapour diffusion by mixing 0.0004 ml reservoir solution with 0.0004 ml protein solution, containing 25 mM HEPES, pH 7.0, 500 mM NaCl, 5% v/v glycerol, 0.025% w/v sodium azide and 2 mM DTT, over a reservoir volume of 0.08 ml, the reservoir solution, containing 5% PEG 1000, 10% glycerol, 30% PEG 600, and 100 mM MES pH 7.5, is supplemented with the ligands at different concentrations, X-ray diffraction structure determination and analysis at 1.5-2.25 A resolution, molecular replacement and modeling
Burkholderia pseudomallei
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.11
vanadate
acting as a substrate mimic, enzyme binding structure, overview
Burkholderia pseudomallei
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.11
2-phospho-D-glycerate
Burkholderia pseudomallei
-
3-phospho-D-glycerate
-
-
r
5.4.2.11
2-phospho-D-glycerate
Burkholderia pseudomallei 1710b
-
3-phospho-D-glycerate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.11
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-R3-pRARE2 cells by nickel affinity chromatography and gel filtration
Burkholderia pseudomallei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.11
2-phospho-D-glycerate
-
726607
Burkholderia pseudomallei
3-phospho-D-glycerate
-
-
-
r
5.4.2.11
2-phospho-D-glycerate
-
726607
Burkholderia pseudomallei 1710b
3-phospho-D-glycerate
-
-
-
r
General Information
EC Number
General Information
Commentary
Organism
5.4.2.11
metabolism
the enzyme catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate as a key step in the glycolytic pathway
Burkholderia pseudomallei
5.4.2.11
additional information
active site residues are Thr21, Gly22, Tyr90, Lys98, Arg114 and Arg115
Burkholderia pseudomallei
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.4.2.11
metabolism
the enzyme catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate as a key step in the glycolytic pathway
Burkholderia pseudomallei
5.4.2.11
additional information
active site residues are Thr21, Gly22, Tyr90, Lys98, Arg114 and Arg115
Burkholderia pseudomallei