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Literature summary extracted from

  • Miyakawa, T.; Hatano, K.I.; Lee, W.C.; Kato, Y.; Sawano, Y.; Yumoto, F.; Nagata, K.; Tanokura, M.
    Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7 (2005), Acta Crystallogr. Sect. F, 61, 828-830.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.10 expressed in Escherichia coli Rosetta(DE3) cells Sulfurisphaera tokodaii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.99.10 sitting drop vapor diffusion method, using 16% (w/v) PEG 10000, 0.1 M bis-Tris pH 5.3 and 0.1 M ammonium acetate Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.10 2-iminobutanoate + H2O Sulfurisphaera tokodaii
-
 2-oxobutanoate + NH3
-
 ?
3.5.99.10 2-iminobutanoate + H2O Sulfurisphaera tokodaii 7
-
 2-oxobutanoate + NH3
-
 ?
3.5.99.10 2-iminopropanoate + H2O Sulfurisphaera tokodaii
-
 pyruvate + NH3
-
 ?
3.5.99.10 2-iminopropanoate + H2O Sulfurisphaera tokodaii 7
-
 pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.10 Sulfurisphaera tokodaii Q973T6
-
-
3.5.99.10 Sulfurisphaera tokodaii 7 Q973T6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.10 Ni-NTA agarose column chromatography, and Superdex 75 gel filtration Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.10 2-iminobutanoate + H2O
-
 Sulfurisphaera tokodaii 2-oxobutanoate + NH3
-
 ?
3.5.99.10 2-iminobutanoate + H2O
-
 Sulfurisphaera tokodaii 7 2-oxobutanoate + NH3
-
 ?
3.5.99.10 2-iminopropanoate + H2O
-
 Sulfurisphaera tokodaii pyruvate + NH3
-
 ?
3.5.99.10 2-iminopropanoate + H2O
-
 Sulfurisphaera tokodaii 7 pyruvate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.99.10 ST0811
-
 Sulfurisphaera tokodaii