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Literature summary extracted from

  • Lipski, A.; Watzlawick, H.; Ravaud, S.; Robert, X.; Rhimi, M.; Haser, R.; Mattes, R.; Aghajari, N.
    Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity (2013), Acta Crystallogr. Sect. D, 69, 298-307.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.11 gene mutB, recombinant expression of wild-type and mutant enzymes Rhizobium sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.11 purified recombinant enzyme mutants R284C in complex with transition-state mimic deoxynojirimycin, and F164L in complex with glucose, sucrose, trehalulose, or isomaltulose, X-ray diffraction structure determination and analysis at 1.7-2.15 A resolution Rhizobium sp.

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.11 F164L site-directed mutagenesis, three-dimensional structure from crystal structure analysis, comparison to the wild-type. The mutant shows hydrolytic activity converting sucrose to glucose and fructose, kinetics Rhizobium sp.
5.4.99.11 R284C site-directed mutagenesis, three-dimensional structure from crystal structure analysis, comparison to the wild-type. The mutant shows hydrolytic activity converting sucrose to glucose and fructose, kinetics. Presence of Mg2+, Ca2+ and Zn2+ ions and glucose have no effect on the activity of the R284C mutant Rhizobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.11 additional information Rhizobium sp. the enzyme catalyzes the isomerization of sucrose (alpha-D-glucosylpyranosyl-1,2-beta-D-fructofuranose) to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as the main products, and produces glucose and fructose in residual amounts because of sucrose hydrolysis ?
-
 ?
5.4.99.11 sucrose Rhizobium sp.
-
 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose main products ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.11 Rhizobium sp. M1E1F6 gene mutB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.11 recombinant wild-type and mutant enzymes Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.11 additional information the enzyme catalyzes the isomerization of sucrose (alpha-D-glucosylpyranosyl-1,2-beta-D-fructofuranose) to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as the main products, and produces glucose and fructose in residual amounts because of sucrose hydrolysis Rhizobium sp. ?
-
 ?
5.4.99.11 sucrose
-
 Rhizobium sp. 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose main products ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.11 MutB
-
 Rhizobium sp.
5.4.99.11 sucrose isomerase
-
 Rhizobium sp.

General Information

EC Number General Information Comment Organism
5.4.99.11 evolution the enzyme belongs to the glycoside hydrolase family 13 (GH13) in the CAZy classification Rhizobium sp.
5.4.99.11 additional information active site structure and substrate and ligand binding structures, overview Rhizobium sp.