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Literature summary extracted from

  • Son, J.; Lee, E.H.; Park, M.; Kim, J.H.; Kim, J.; Kim, S.; Jeon, Y.H.; Hwang, K.Y.
    Conformational changes in human prolyl-tRNA synthetase upon binding of the substrates proline and ATP and the inhibitor halofuginone (2013), Acta Crystallogr. Sect. D, 69, 2136-2145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.15 complexed with ATP and L-proline or halofuginone, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate, 1.0 M sodium citrate dibasic trihydrate pH 6.5 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 F1097A the mutant shows strongly decreased aminoacylation activity compared to the wild type enzyme Homo sapiens
6.1.1.15 F1097W the mutant shows increased aminoacylation activity compared to the wild type enzyme Homo sapiens
6.1.1.15 R1152K the mutant shows increased aminoacylation activity compared to the wild type enzyme Homo sapiens
6.1.1.15 R1152L the mutant shows strongly decreased aminoacylation activity compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 halofuginone strong inhibition at 0.001 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Homo sapiens
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Homo sapiens P07814
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 Ni-NTA affinity column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Homo sapiens AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
 Homo sapiens
6.1.1.15 PRS
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Homo sapiens