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Literature summary extracted from

  • Hoeppner, A.; Thomas F, Rueppel, A.; Hensel, R.; Blankenfeldt W, Bayer P, Faust A.
    Structure of the corrinoid:coenzyme M methyltransferase MtaA from Methanosarcina mazei (2012), Acta Crystallogr. Sect. D, 68, 1549-1557.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.247
-
 Methanosarcina mazei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.247 crystals of His6-tagged MtaA are obtained at room temperature by the hanging-drop vapour-diffusion method. Hexagonal-shaped crystals of the selenomethionine derivative are obtained by the sitting-drop vapour-diffusion method. The crystal structures of MtaA in a substrate-free but Zn2+-bound state and in complex with Zn2+ and coenzyme M (HS-CoM) are reported at resolutions of 1.8 and 2.1 A Methanosarcina mazei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.90 Zinc dependent on Methanosarcina mazei
2.1.1.247 Zn2+ the Zn2+ ion in the active site of MtaA is coordinated tetrahedrally via His240, Cys242 and Cys319. In the substrate-free form the fourth ligand is Glu263 Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.90 methanol + a [Co(I) methanol-specific corrinoid protein] Methanosarcina mazei
-
 a [methyl-Co(III) methanol-specific corrinoid protein] + H2O
-
 ?
2.1.1.90 methanol + a [Co(I) methanol-specific corrinoid protein] Methanosarcina mazei DSM 3647
-
 a [methyl-Co(III) methanol-specific corrinoid protein] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.90 Methanosarcina mazei Q8PXZ6
-
-
2.1.1.247 Methanosarcina mazei Q8PXZ6
-
-
2.1.1.247 Methanosarcina mazei DSM 3647 Q8PXZ6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.90 methanol + a [Co(I) methanol-specific corrinoid protein]
-
 Methanosarcina mazei a [methyl-Co(III) methanol-specific corrinoid protein] + H2O
-
 ?
2.1.1.90 methanol + a [Co(I) methanol-specific corrinoid protein]
-
 Methanosarcina mazei DSM 3647 a [methyl-Co(III) methanol-specific corrinoid protein] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.90 methanol-coenzyme M-methyltransferase complex
-
 Methanosarcina mazei
2.1.1.90 MtaB part of the methanol-coenzyme M-methyltransferase complex Methanosarcina mazei
2.1.1.247 corrinoid:coenzyme M methyltransferase
-
 Methanosarcina mazei
2.1.1.247 MM_1070 locus name Methanosarcina mazei
2.1.1.247 mtaA
-
 Methanosarcina mazei