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Literature summary extracted from

  • Peng, N.; Zhong, Y.; Zhang, Q.; Zheng, M.; Zhao, W.; Jiang, H.; Yang, C.; Guo, X.; Zhao, G.
    Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO(2) assimilation (2012), Acta Biochim. Biophys. Sin., 44, 692-702.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.3 expressed in Escherichia coli as a His-tagged fusion protein Leptospira interrogans

Protein Variants

EC Number Protein Variants Comment Organism
6.4.1.3 A431C kcat (propionyl-CoA) decreased compared to wild-type, Km (propionyl-CoA) increased compared to wild-type. kcat (acetyl-CoA) increased compared to wild-type, Km (propionyl-CoA) similar to wild-type Leptospira interrogans
6.4.1.3 A431D mutant enzyme shows no activity Leptospira interrogans
6.4.1.3 A431I kcat (propionyl-CoA) decreased compared to wild-type, Km (propionyl-CoA) increased compared to wild-type. kcat (acetyl-CoA) increased compared to wild-type, Km (propionyl-CoA) similar to wild-type Leptospira interrogans
6.4.1.3 A431L mutant enzyme shows no activity Leptospira interrogans
6.4.1.3 Y430H/A431I mutant enzyme shows no activity Leptospira interrogans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.3 0.1 1 propionyl-CoA pH 8, 30°C, wild-type Leptospira interrogans
6.4.1.3 0.24
-
propionyl-CoA pH 8, 30°C, mutant A431C Leptospira interrogans
6.4.1.3 1.3
-
propionyl-CoA pH 8, 30°C, mutant A431I Leptospira interrogans
6.4.1.3 4.6
-
acetyl-CoA pH 8, 30°C, wild-type Leptospira interrogans
6.4.1.3 4.8
-
acetyl-CoA pH 8, 30°C, mutant A431C Leptospira interrogans
6.4.1.3 5
-
acetyl-CoA pH 8, 30°C, mutant A431I Leptospira interrogans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.3 669000
-
alpha4beta4 quaternary structure Leptospira interrogans

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.3 Leptospira interrogans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.3 using Ni-NTA chromatography Leptospira interrogans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.3 ATP + acetyl-CoA + HCO3-
-
Leptospira interrogans ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + propionyl-CoA + HCO3- preferred substrate Leptospira interrogans ADP + phosphate + (S)-methylmalonyl-CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
6.4.1.3 LA_2736-LA_2735
-
Leptospira interrogans
6.4.1.3 PCC
-
Leptospira interrogans
6.4.1.3 pccBC
-
Leptospira interrogans
6.4.1.3 Propionyl-CoA carboxylase
-
Leptospira interrogans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.3 30
-
assay at Leptospira interrogans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4.1.3 0.47
-
propionyl-CoA pH 8, 30°C, mutant A431I Leptospira interrogans
6.4.1.3 0.66
-
acetyl-CoA pH 8, 30°C, wild-type Leptospira interrogans
6.4.1.3 1.4
-
acetyl-CoA pH 8, 30°C, mutant A431I Leptospira interrogans
6.4.1.3 1.46
-
propionyl-CoA pH 8, 30°C, mutant A431C Leptospira interrogans
6.4.1.3 2.86
-
propionyl-CoA pH 8, 30°C, wild-type Leptospira interrogans
6.4.1.3 4
-
acetyl-CoA pH 8, 30°C, mutant A431C Leptospira interrogans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.3 8
-
assay at Leptospira interrogans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.4.1.3 0.144
-
acetyl-CoA pH 8, 30°C, wild-type Leptospira interrogans
6.4.1.3 0.274
-
acetyl-CoA pH 8, 30°C, mutant A431I Leptospira interrogans
6.4.1.3 0.367
-
propionyl-CoA pH 8, 30°C, mutant A431I Leptospira interrogans
6.4.1.3 0.826
-
acetyl-CoA pH 8, 30°C, mutant A431C Leptospira interrogans
6.4.1.3 6.09
-
propionyl-CoA pH 8, 30°C, mutant A431C Leptospira interrogans
6.4.1.3 26
-
propionyl-CoA pH 8, 30°C, wild-type Leptospira interrogans