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Literature summary extracted from

  • Huang, J.; Bathena, S.P.; Tong, J.; Roth, M.; Hagenbuch, B.; Alnouti, Y.
    Kinetic analysis of bile acid sulfation by stably expressed human sulfotransferase 2A1 (SULT2A1) (2010), Xenobiotica, 40, 184-194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.14 expressed in HEK-293 cells Homo sapiens
2.8.2.14 expressed in HEK293 cells Homo sapiens

General Stability

EC Number General Stability Organism
2.8.2.14 SULT2A1 activity is consistently higher using NaH2PO4 buffer in a pH range from 5.5 to 6.5 Homo sapiens
2.8.2.14 SULT2A1 activity is consistently highest using NaH2PO4 buffer in a pH range from 5.5 to 6.5 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.14 0.00186
-
 taurine conjugated lithocholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.00192
-
 glycine conjugated lithocholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.00202
-
 lithocholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
2.8.2.14 0.00202
-
 lithocholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.00383
-
 dehydroepiandrosterone apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
2.8.2.14 0.00383
-
 dehydroepiandrosterone apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.00886
-
 deoxycholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
2.8.2.14 0.00886
-
 deoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.00993
-
 taurine conjugated ursodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.01286
-
 Ursodeoxycholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
2.8.2.14 0.01286
-
 ursodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.01566
-
 chenodeoxycholic acid apparent value, at pH 5.5 and 7.4, at 37°C Homo sapiens
2.8.2.14 0.01566
-
 chenodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.01836
-
 taurine conjugated deoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.04509
-
 taurine conjugated chenodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.04899
-
 glycine conjugated chenodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.04957
-
 glycine conjugated ursodeoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens
2.8.2.14 0.05459
-
 glycine conjugated deoxycholate apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.14 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.14 Mg2+ the addition of 2 mM MgCl2 to the reaction causes a 20% increase in sulfation activity, whereas higher concentrations of Mg2+ inhibit enzyme activity Homo sapiens
2.8.2.14 Mg2+ the presence of MgCl2 is not required for the SULT2A1 enzyme activity. However, the addition of 2 mM MgCl2 to the reaction causes a 20% increase in sulfation activity, whereas higher concentrations of Mg2+ inhibit SULT2A1 activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.14 33780
-
 x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence Homo sapiens
2.8.2.14 34000
-
 x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.14 3'-phosphoadenylylsulfate + chenodeoxycholic acid Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + cholic acid Homo sapiens lowest affinity to sulfation by SULT2A1 adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + dehydroepiandrosterone Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + deoxycholic acid Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + lithocholic acid Homo sapiens highest affinity to sulfation by SULT2A1 adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + ursodeoxycholic acid Homo sapiens
-
 adenosine 3',5'-bisphosphate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.14 Homo sapiens
-
-
-
2.8.2.14 Homo sapiens Q06520
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.14 3'-phosphoadenylyl-sulfate + chenodeoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + deoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + glycine conjugated chenodeoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + glycine conjugated deoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + glycine conjugated lithocholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + glycine conjugated ursodeoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + lithocholate highest affinity substrate Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + taurine conjugated chenodeoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + taurine conjugated deoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + taurine conjugated lithocholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + taurine conjugated ursodeoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylyl-sulfate + ursodeoxycholate
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + chenodeoxycholic acid
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + cholic acid lowest affinity to sulfation by SULT2A1 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + deoxycholic acid
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + lithocholic acid highest affinity to sulfation by SULT2A1 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 3'-phosphoadenylylsulfate + ursodeoxycholic acid
-
 Homo sapiens adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.14 additional information no activity with cholate, glycine conjugated cholate and taurine conjugated cholate Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.14 ? x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.8.2.14 dehydroepiandrosterone-SULT
-
 Homo sapiens
2.8.2.14 DHEA-SULT
-
 Homo sapiens
2.8.2.14 hydroxysteroid sulfotransferase
-
 Homo sapiens
2.8.2.14 sulfotransferase 2A1
-
 Homo sapiens
2.8.2.14 SULT2A1
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.14 5.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.14 4 9 a more than 10fold increase in enzyme activity is observed with increasing the pH from 4.0 to 5.5, while further increase in pH in 10 mM Tris-HCl buffer is associated with a decline in the enzyme activity Homo sapiens
2.8.2.14 4 9 more than a 10fold increase in SULT2A1 activity is observed with increasing the pH from 4.0 to 5.5, while a further increase in pH in 10 mM Tris-HCl buffer (up to pH 9-0) is associated with a 50% decline in the optimum enzyme activity Homo sapiens