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Literature summary extracted from

  • Lee, J.I.; Kim, Y.W.
    Characterization of amine oxidases from Arthrobacter aurescens and application for determination of biogenic amines (2013), World J. Microbiol. Biotechnol., 29, 673-682.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21 expressed in Escherichia coli BL21(DE3) cells Paenarthrobacter aurescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 2-Phenylethylamine substrate inhibition Paenarthrobacter aurescens
1.4.3.21 histamine substrate inhibition; substrate inhibition Paenarthrobacter aurescens
1.4.3.21 additional information the enzyme activity is not significantly affected in the presence of 5 mM EDTA; the enzyme activity is not significantly affected in the presence of 5 mM EDTA Paenarthrobacter aurescens
1.4.3.21 tyramine substrate inhibition; substrate inhibition Paenarthrobacter aurescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.0055
-
 2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.012
-
 2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.02
-
 tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.03
-
 tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.41
-
 histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.88
-
 histamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 7.04
-
 spermidine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 12.7
-
 cadaverine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 Cu2+ required for activity, after overnight incubation with 0.1 mM CuSO4, isoform AMAO3 shows slightly enhanced activity (114% of untreated enzyme activity) Paenarthrobacter aurescens
1.4.3.21 Cu2+ required for activity, after overnight incubation with 0.1 mM CuSO4, there is no change in the activity of isoform AMAO2 Paenarthrobacter aurescens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Paenarthrobacter aurescens A1R2C3 isoform AMAO2
-
1.4.3.21 Paenarthrobacter aurescens A1RDD3 isoform AMAO3
-
1.4.3.21 Paenarthrobacter aurescens TC1 A1R2C3 isoform AMAO2
-
1.4.3.21 Paenarthrobacter aurescens TC1 A1RDD3 isoform AMAO3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21 nickel affinity column chromatography Paenarthrobacter aurescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 2-phenylethylamine + H2O + O2
-
 Paenarthrobacter aurescens ?
-
 ?
1.4.3.21 2-phenylethylamine + H2O + O2 best substrate Paenarthrobacter aurescens phenylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 cadaverine + H2O + O2
-
 Paenarthrobacter aurescens ?
-
 ?
1.4.3.21 cadaverine + H2O + O2
-
 Paenarthrobacter aurescens TC1 ?
-
 ?
1.4.3.21 histamine + H2O + O2
-
 Paenarthrobacter aurescens ?
-
 ?
1.4.3.21 histamine + H2O + O2
-
 Paenarthrobacter aurescens TC1 ?
-
 ?
1.4.3.21 putrescine + H2O + O2
-
 Paenarthrobacter aurescens ?
-
 ?
1.4.3.21 putrescine + H2O + O2
-
 Paenarthrobacter aurescens TC1 ?
-
 ?
1.4.3.21 spermidine + H2O + O2
-
 Paenarthrobacter aurescens ?
-
 ?
1.4.3.21 spermidine + H2O + O2
-
 Paenarthrobacter aurescens TC1 ?
-
 ?
1.4.3.21 tyramine + H2O + O2
-
 Paenarthrobacter aurescens (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 tyramine + H2O + O2
-
 Paenarthrobacter aurescens TC1 (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 AMAO2 isoform Paenarthrobacter aurescens
1.4.3.21 AMAO3 isoform Paenarthrobacter aurescens
1.4.3.21 copper-containing monoamine oxidase
-
 Paenarthrobacter aurescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.21 35
-
 isoform AMAO3 Paenarthrobacter aurescens
1.4.3.21 55
-
 isoform AMAO2 Paenarthrobacter aurescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 0.0429
-
 spermidine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.0619
-
 cadaverine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.46
-
 histamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 1.6
-
 tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 1.72
-
 2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 3.95
-
 tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 5.12
-
 2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 5.91
-
 histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.21 6.5
-
 isoform AMAO2 Paenarthrobacter aurescens
1.4.3.21 7
-
 isoform AMAO3 Paenarthrobacter aurescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
 Paenarthrobacter aurescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.21 0.84
-
 2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 1.08
-
 tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 1.41
-
 2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 2.93
-
 tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 3.51
-
 histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.21 0.15
-
 putrescine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 0.52
-
 histamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 14.6
-
 histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 82.3
-
 tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 133
-
 tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 314
-
 2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.4.3.21 429
-
 2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens