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Literature summary extracted from

  • Daassi, D.; Zouari-Mechichi, H.; Prieto, A.; Martinez, M.J.; Nasri, M.; Mechichi, T.
    Purification and biochemical characterization of a new alkali-stable laccase from Trametes sp. isolated in Tunisia: role of the enzyme in olive mill waste water treatment (2013), World J. Microbiol. Biotechnol., 29, 2145-2155.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 DNA and amino acid sequence determination and analysis Trametes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Cd2+
-
 Trametes sp.
1.10.3.2 EDTA 45% inhibition at 10 mM, 10% at 1 mM Trametes sp.
1.10.3.2 Fe2+
-
 Trametes sp.
1.10.3.2 L-cysteine 90% inhibition at 10 mM, 70% at 1 mM Trametes sp.
1.10.3.2 Mn2+
-
 Trametes sp.
1.10.3.2 NaN3 complete inhibition at 0.1 mM Trametes sp.
1.10.3.2 SDS 40% inhibition at 10 mM, 10% at 1 mM Trametes sp.
1.10.3.2 Zn2+
-
 Trametes sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Trametes sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ca2+ activates Trametes sp.
1.10.3.2 Cu2+ activates Trametes sp.
1.10.3.2 Fe3+ activates Trametes sp.
1.10.3.2 Mg2+ activates Trametes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 61000
-
 x * 61000, SDS-PAGE Trametes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 4 catechol + O2 Trametes sp.
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2 Trametes sp. BS54
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes sp.
-
 isolated from decayed acacia wood from Northwest of Tunisia
-
1.10.3.2 Trametes sp. BS54
-
 isolated from decayed acacia wood from Northwest of Tunisia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native extracellular laccase 10fold by ultrafilatration, anion exchange chromatography, and again ultrafiltration, to homogeneity Trametes sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Trametes sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 12.8
-
 pH 4.0, 60°C, purified enzyme, substrate 1,2,3-trihydroxybenzene Trametes sp.
1.10.3.2 17
-
 pH 4.0, 60°C, purified enzyme, substrate 3-aminobenzoic acid Trametes sp.
1.10.3.2 51.4
-
 pH 4.0, 60°C, purified enzyme, substrate catechol Trametes sp.
1.10.3.2 61.2
-
 pH 4.0, 60°C, purified enzyme, substrate guaiacol Trametes sp.
1.10.3.2 71.6
-
 pH 4.0, 60°C, purified enzyme, substrate toluidine Trametes sp.
1.10.3.2 86.5
-
 pH 4.0, 60°C, purified enzyme, substrate 2,6-dimethoxyphenol Trametes sp.
1.10.3.2 86.5
-
 pH 4.0, 60°C, purified enzyme, substrate guaiacol Trametes sp.
1.10.3.2 112.6
-
 pH 4.0, 60°C, purified enzyme, substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Trametes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4 1,2,3-trihydroxybenzene + O2 i.e pyrogallol Trametes sp. ?
-
 ?
1.10.3.2 4 1,2,3-trihydroxybenzene + O2 i.e pyrogallol Trametes sp. BS54 ?
-
 ?
1.10.3.2 4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes sp. ?
-
 ?
1.10.3.2 4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes sp. BS54 ?
-
 ?
1.10.3.2 4 2,6-dimethoxyphenol + O2
-
 Trametes sp. ?
-
 ?
1.10.3.2 4 2,6-dimethoxyphenol + O2
-
 Trametes sp. BS54 ?
-
 ?
1.10.3.2 4 3-aminobenzoic acid + O2
-
 Trametes sp. ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes sp. 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes sp. BS54 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 guaiacol + O2
-
 Trametes sp. ?
-
 ?
1.10.3.2 4 toluidine + O2
-
 Trametes sp. ?
-
 ?
1.10.3.2 additional information substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid Trametes sp. ?
-
 ?
1.10.3.2 additional information substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid Trametes sp. BS54 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 61000, SDS-PAGE Trametes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 60
-
-
 Trametes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 55
-
 loss of 50% activity after 90 min Trametes sp.
1.10.3.2 65
-
 loss of 50% activity after 30 min Trametes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4
-
-
 Trametes sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4 9 the purified laccase is more stable at alkaline pH of 7.0-9.0 after 24 h than at its optimum pH of 4.0 Trametes sp.