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Literature summary extracted from

  • Niwas, R.; Singh, V.; Singh, R.; Tripathi, D.; Tripathi, C.K.
    Production, purification and characterization of cholesterol oxidase from a newly isolated Streptomyces sp. (2013), World J. Microbiol. Biotechnol., 29, 2077-2085.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.6 ethanol activates 1.6fold at 10% Streptomyces sp.
1.1.3.6 Isopropanol activates 2.3fold at 10% Streptomyces sp.
1.1.3.6 methanol activates 1.15fold at 10% Streptomyces sp.
1.1.3.6 Triton X-100 acivates 3.5fold at 0.5% Streptomyces sp.
1.1.3.6 Triton X-114 acivates 2fold at 0.5% Streptomyces sp.
1.1.3.6 Tween 80 acivates 1.5fold at 0.5% Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 2-mercaptoethanol
-
 Streptomyces sp.
1.1.3.6 acetone inhibits 43% at 10% Streptomyces sp.
1.1.3.6 Ba2+ inhibits 21.4% at 5 mM Streptomyces sp.
1.1.3.6 Benzene inhibits 20% at 10% Streptomyces sp.
1.1.3.6 chloroform inhibits 80% at 10% Streptomyces sp.
1.1.3.6 dithiothreitol
-
 Streptomyces sp.
1.1.3.6 DMSO inhibits 5% at 10% Streptomyces sp.
1.1.3.6 Hg2+ inhibits 21.8% at 5 mM Streptomyces sp.
1.1.3.6 Mn2+ inhibits 4.5% at 5 mM Streptomyces sp.
1.1.3.6 Sodium dodecyl sulfate inactivates the enzyme Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.1013
-
 cholesterol pH 7.0, 37°C Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.6 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.6 Co2+ activates 3.6% at 5 mM Streptomyces sp.
1.1.3.6 Cu2+ activates 3fold at 5 mM Streptomyces sp.
1.1.3.6 additional information no or poor effects at 5 mM by Ca2+, Zn2+, Mg2+, and Fe2+ Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 62000
-
 x * 62000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Streptomyces sp.
-
 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2 Streptomyces sp. R-6
-
 cholest-4-en-3-one + H2O2
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.3.6 additional information the enzyme shows moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increases in presence of isopropanol and ethanol Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Streptomyces sp.
-
-
-
1.1.3.6 Streptomyces sp. R-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6 native extracellular enzyme 14.3fold by ammonium sulfate fractionation and affinity chromatography Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.6 additional information effect of carbon and nitrogen sources on COD production, highest activity on lactose and sucrose and on yeast extract, respectively, overview Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 21.8
-
 purified enzyme, pH 7.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Streptomyces sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces sp. R-6 cholest-4-en-3-one + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.6 ? x * 62000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 COD
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 37
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 20 60 activity range, profile overview Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 4 10 activity range, profile overview Streptomyces sp.