Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Freixo, M.d.o..R.; Karmali, A.; Arteiro, J.M.
    Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace (2012), World J. Microbiol. Biotechnol., 28, 245-254.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0172
-
 2-dianisidine pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.0552
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 4
-
 guaiacol pH 6.0, 25°C Pleurotus ostreatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ the enzyme is a multi-copper enzyme Pleurotus ostreatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 71000
-
 native PAGE and gel filtration Pleurotus ostreatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 additional information Pleurotus ostreatus the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Pleurotus ostreatus
-
 grown on tomato pomace, several isoyzmes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 method development for one-step affinity chromatographic purifcation, 46fold purification on 1,4-butanediol diglycidyl ether affinity resin, analysis of affinity chromatographic matrices containing either urea, acetamide, ethanolamine, or iminodiacetic acid, and best with 1,4-butanediol diglycidyl ether, as affinity ligands for enzyme purification from fermentation broth Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information cultivation of Pleurotus ostreatus on tomato pomace medium Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 17.8
-
 purified native enzyme, pH 6.0, 25°C Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus ?
-
 ?
1.10.3.2 2-dianisidine + O2
-
 Pleurotus ostreatus ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Pleurotus ostreatus ?
-
 ?
1.10.3.2 additional information the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water Pleurotus ostreatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 52000-55000, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Pleurotus ostreatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 3.9
-
 guaiacol pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 20
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 20.7
-
 2-dianisidine pH 6.0, 25°C Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6
-
 assay at Pleurotus ostreatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.0172
-
 2-dianisidine pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.0552
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 4
-
 guaiacol pH 6.0, 25°C Pleurotus ostreatus