Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toh, H.; Wang, S.; Chang, S.; Chu, F.
    Molecular cloning and characterization of flavonol synthase in Acacia confusa (2013), Tree Genet. Genomes, 9, 85-92.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.6 gene AcFLS, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged FLS protein in Escherichia coli strain BL21-CodonPlus-RIL Acacia confusa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.6 Fe2+ dependent on, the enzyme contains a ferrous iron (II) binding residue motif, i.e. the HxDxnH motif consisting of His217, Asp219, and His273 Acacia confusa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.6 38000
-
 x * 38000, about, sequence calculation Acacia confusa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.6 (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 Acacia confusa
-
 quercetin + succinate + CO2 + H2O
-
 ?
1.14.20.6 dihydromyricetin + 2-oxoglutarate + O2 Acacia confusa
-
 myricetin + succinate + CO2 + H2O
-
 ?
1.14.20.6 naringenin + 2-oxoglutarate + O2 Acacia confusa
-
 kaempferol + succinate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.6 Acacia confusa H6V8U4 gene AcFLS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.6 recombinant His-tagged FLS protein from Escherichia coli strain BL21-CodonPlus-RIL by nickel affinity chromatography Acacia confusa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.6 flower
-
 Acacia confusa
-
1.14.20.6 leaf
-
 Acacia confusa
-
1.14.20.6 seedling
-
 Acacia confusa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.20.6 0.15
-
 purified recombinant His-tagged enzyme, substrate dihydroquercetin, pH 6.8, 25°C Acacia confusa
1.14.20.6 0.154
-
 purified recombinant His-tagged enzyme, substrate dihydromyricetin, pH 6.8, 25°C Acacia confusa
1.14.20.6 0.163
-
 purified recombinant His-tagged enzyme, substrate naringenin, pH 6.8, 25°C Acacia confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.6 (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2
-
 Acacia confusa quercetin + succinate + CO2 + H2O
-
 ?
1.14.20.6 dihydromyricetin + 2-oxoglutarate + O2
-
 Acacia confusa myricetin + succinate + CO2 + H2O
-
 ?
1.14.20.6 additional information substrates are bound with similar affinity Acacia confusa ?
-
 ?
1.14.20.6 naringenin + 2-oxoglutarate + O2
-
 Acacia confusa kaempferol + succinate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.20.6 ? x * 38000, about, sequence calculation Acacia confusa
1.14.20.6 More FLS protein structure modeling, overview Acacia confusa

Synonyms

EC Number Synonyms Comment Organism
1.14.20.6 FLS
-
 Acacia confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.20.6 25
-
 assay at Acacia confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.6 6.8
-
 assay at Acacia confusa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.20.6 Acacia confusa sequence calculation
-
 5.7

Expression

EC Number Organism Comment Expression
1.14.20.6 Acacia confusa wounding by cutting leaves induces maximum AcFLS mRNA accumulation 6-12 h after treatment up

General Information

EC Number General Information Comment Organism
1.14.20.6 evolution the enzyme belongs to the the 2-oxoglutarate-Fe(II) dioxygenase superfamily Acacia confusa
1.14.20.6 additional information FLS protein structure modeling, overview. The structures of two major flavonols, i.e. melanoxetin or transilitin, found in Acacia confusa heartwood are different from common flavonoids Acacia confusa