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Literature summary extracted from

  • Wan, S.; Mottiar, Y.; Johnson, A.M.; Goto, K.; Altosaar, I.
    Expression of the nos operon proteins from Pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase (2012), Transgenic Res., 21, 593-603.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.7.2.4 agriculture expression of both the senzyme-coding gene nosZ and the mega-cassette of five coding sequences nosFLZDY in Nicotiana tabacum leads to active recombinant N2OR. Extracts from both types of transgenic plants exhibit N2O-reducing activity. The single-gene strategy produces higher reductase capability than the whole-operon approach. Bacterial nitrous oxide reductase expressed in plants could convert N2O into inert N2 without involvement of other Nos proteins Pseudomonas stutzeri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.4 expression in Nicotiana tabacum Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Pseudomonas stutzeri P19573
-
-
1.7.2.4 Pseudomonas stutzeri ATCC 14405 P19573
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 453
-
 enzyme purified from transgenic Nicotiana tabacum, pH 7.1, 37°C Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 N2O + reduced methyl viologen
-
 Pseudomonas stutzeri N2 + oxidized methyl viologen
-
 ?
1.7.2.4 N2O + reduced methyl viologen
-
 Pseudomonas stutzeri ATCC 14405 N2 + oxidized methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.4 NosZ
-
 Pseudomonas stutzeri