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Literature summary extracted from

  • Guedez, G.; Hipp, K.; Windeisen, V.; Derrer, B.; Gengenbacher, M.; Böttcher, B.; Sinning, I.; Kappes, B.; Tews, I.
    Assembly of the eukaryotic PLP-synthase complex from Plasmodium and activation of the Pdx1 enzyme (2012), Structure, 20, 172-184.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL Plasmodium falciparum
4.3.3.6 His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL Plasmodium berghei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.6 chimeric complex of Pdx2 and Pdx1 from Plasmodium berghei Plasmodium falciparum
4.3.3.6 Pdx1, chimeric complex of Pdx1 and Pdx2 from Plasmodium falciparum Plasmodium berghei

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.6 H196N Pdx2, catalytically inactive Plasmodium falciparum
4.3.3.6 H199N Pdx2 Plasmodium berghei
4.3.3.6 L82A Pdx1 Plasmodium berghei
4.3.3.6 M103A Pdx1 Plasmodium berghei
4.3.3.6 M103F Pdx1 Plasmodium berghei
4.3.3.6 M148L Pdx1 Plasmodium berghei
4.3.3.6 M19V Pdx1 Plasmodium berghei
4.3.3.6 M46I Pdx1 Plasmodium berghei

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Plasmodium berghei A0A509AM71
-
-
4.3.3.6 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6 immobilized metal ion affinity chromatography (Ni2+), gel filtration Plasmodium falciparum
4.3.3.6 immobilized metal ion affinity chromatography (Ni2+), gel filtration Plasmodium berghei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.6 0.00025
-
L82A mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.00031
-
M103A mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.00042
-
M103A mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0007
-
Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium falciparum
4.3.3.6 0.0008
-
M148L mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0009
-
Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0009
-
Pdx1/Plasmodium berghei Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium falciparum
4.3.3.6 0.0009
-
Plasmodium falciparum Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0011
-
Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0012
-
L82A mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0013
-
Pdx1/Plasmodium falciparum Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0013
-
Plasmodium berghei Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium falciparum
4.3.3.6 0.0015
-
M46I mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0018
-
M19V mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.002
-
M19V mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0028
-
M46I mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.0037
-
M148L mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C Plasmodium berghei
4.3.3.6 0.087
-
M19V mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.115
-
L82A mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.126
-
M103F mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.15
-
Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.175
-
Pdx1/Plasmodium berghei Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium falciparum
4.3.3.6 0.175
-
Plasmodium falciparum Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.178
-
M103A mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.229
-
M148L mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.233
-
Pdx1/Plasmodium falciparum Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei
4.3.3.6 0.233
-
Plasmodium berghei Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium falciparum
4.3.3.6 0.26
-
Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium falciparum
4.3.3.6 0.284
-
M46I mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C Plasmodium berghei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine
-
Plasmodium falciparum pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
?
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine
-
Plasmodium berghei pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
?
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ in the absence of Pdx2 Plasmodium falciparum pyridoxal 5'-phosphate + 4 H2O + phosphate
-
?
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ in the absence of Pdx2 Plasmodium berghei pyridoxal 5'-phosphate + 4 H2O + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
4.3.3.6 homododecamer Pdx1, random association pattern of up to 12 Pdx2 subunits to the Pdx1 dodecamer Plasmodium falciparum
4.3.3.6 homododecamer Pdx1, random association pattern of up to 12 Pdx2 subunits to the Pdx1 dodecamer Plasmodium berghei

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 PLP-synthase complex
-
Plasmodium falciparum
4.3.3.6 PLP-synthase complex
-
Plasmodium berghei