EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.6 | His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL | Plasmodium falciparum |
4.3.3.6 | His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL | Plasmodium berghei |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.6 | chimeric complex of Pdx2 and Pdx1 from Plasmodium berghei | Plasmodium falciparum |
4.3.3.6 | Pdx1, chimeric complex of Pdx1 and Pdx2 from Plasmodium falciparum | Plasmodium berghei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.6 | H196N | Pdx2, catalytically inactive | Plasmodium falciparum |
4.3.3.6 | H199N | Pdx2 | Plasmodium berghei |
4.3.3.6 | L82A | Pdx1 | Plasmodium berghei |
4.3.3.6 | M103A | Pdx1 | Plasmodium berghei |
4.3.3.6 | M103F | Pdx1 | Plasmodium berghei |
4.3.3.6 | M148L | Pdx1 | Plasmodium berghei |
4.3.3.6 | M19V | Pdx1 | Plasmodium berghei |
4.3.3.6 | M46I | Pdx1 | Plasmodium berghei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.6 | Plasmodium berghei | A0A509AM71 | - |
- |
4.3.3.6 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.6 | immobilized metal ion affinity chromatography (Ni2+), gel filtration | Plasmodium falciparum |
4.3.3.6 | immobilized metal ion affinity chromatography (Ni2+), gel filtration | Plasmodium berghei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.3.6 | 0.00025 | - |
L82A mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.00031 | - |
M103A mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.00042 | - |
M103A mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0007 | - |
Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium falciparum |
4.3.3.6 | 0.0008 | - |
M148L mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0009 | - |
Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0009 | - |
Pdx1/Plasmodium berghei Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium falciparum |
4.3.3.6 | 0.0009 | - |
Plasmodium falciparum Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0011 | - |
Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0012 | - |
L82A mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0013 | - |
Pdx1/Plasmodium falciparum Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0013 | - |
Plasmodium berghei Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium falciparum |
4.3.3.6 | 0.0015 | - |
M46I mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0018 | - |
M19V mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.002 | - |
M19V mutant Pdx1, NH4Cl as NH3+ donor, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0028 | - |
M46I mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.0037 | - |
M148L mutant Pdx1/Pdx2 complex, pyridoxal 5'-phosphate synthesis, pH 8, 37°C | Plasmodium berghei |
4.3.3.6 | 0.087 | - |
M19V mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.115 | - |
L82A mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.126 | - |
M103F mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.15 | - |
Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.175 | - |
Pdx1/Plasmodium berghei Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium falciparum |
4.3.3.6 | 0.175 | - |
Plasmodium falciparum Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.178 | - |
M103A mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.229 | - |
M148L mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.233 | - |
Pdx1/Plasmodium falciparum Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
4.3.3.6 | 0.233 | - |
Plasmodium berghei Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium falciparum |
4.3.3.6 | 0.26 | - |
Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium falciparum |
4.3.3.6 | 0.284 | - |
M46I mutant Pdx1/Pdx2 complex, glutaminase activity, pH 8, 30°C | Plasmodium berghei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.6 | D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine | - |
Plasmodium falciparum | pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? | |
4.3.3.6 | D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine | - |
Plasmodium berghei | pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate | - |
? | |
4.3.3.6 | D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ | in the absence of Pdx2 | Plasmodium falciparum | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? | |
4.3.3.6 | D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ | in the absence of Pdx2 | Plasmodium berghei | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.3.6 | homododecamer | Pdx1, random association pattern of up to 12 Pdx2 subunits to the Pdx1 dodecamer | Plasmodium falciparum |
4.3.3.6 | homododecamer | Pdx1, random association pattern of up to 12 Pdx2 subunits to the Pdx1 dodecamer | Plasmodium berghei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.6 | PLP-synthase complex | - |
Plasmodium falciparum |
4.3.3.6 | PLP-synthase complex | - |
Plasmodium berghei |