Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoval-Sanchez, B.; Pardo, J.P.; Rodriguez-Zavala, J.S.
    New insights into the half-of-the-sites reactivity of human aldehyde dehydrogenase 1A1 (2013), Proteins, 81, 1330-1339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 the GFP-tagged enzyme is expressed in Escherichia coli BL21(DE3) pLysS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 D80G/S82A the unstable mutant starts to denature at urea concentrations below 0.5 M, reaching half of the denaturation at 1.2 M urea Homo sapiens

General Stability

EC Number General Stability Organism
1.2.1.3 urea concentrations higher than 4.5 M almost completely denature wild type isoform ALDH1A1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 acrolein
-
 Homo sapiens
1.2.1.3 arsenite 47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4 Homo sapiens
1.2.1.3 Chloroacetaldehyde 86% residual activity at 1 mM chloroacetaldehyde at pH 9.5 Homo sapiens
1.2.1.3 Mg2+ wild type enzyme activity is inhibited (64%) by Mg2+ at pH 7.4, whereas the GFP-tagged enzyme activity is not affected by Mg2+ Homo sapiens
1.2.1.3 additional information not influenced by acetaldehyde Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0043
-
 malondialdehyde wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
1.2.1.3 0.009
-
 acrolein wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
1.2.1.3 0.011
-
 NAD+ wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
1.2.1.3 0.012
-
 propionaldehyde wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
1.2.1.3 0.136
-
 NAD+ mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
1.2.1.3 0.189
-
 propionaldehyde mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens
1.2.1.3 0.595
-
 glyceraldehyde wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 50000
-
 4 * 50000, SDS-PAGE Homo sapiens
1.2.1.3 85000
-
 2 * 85000, GFP-tagged enzyme, SDS-PAGE Homo sapiens
1.2.1.3 170000
-
 GFP-tagged enzyme, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 nickel chelating Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acrolein + NAD+ + H2O
-
 Homo sapiens ? + NADH + H+
-
 ?
1.2.1.3 glyceraldehyde + NAD+ + H2O
-
 Homo sapiens glycerate + NADH + H+
-
 ?
1.2.1.3 malondialdehyde + NAD+ + H2O
-
 Homo sapiens ? + NADH + H+
-
 ?
1.2.1.3 additional information no activity with aromatic aldehydes such as benzaldehyde, nitrobenzaldehyde, methoxybenzaldehyde, or phenylacetaldehyde Homo sapiens ?
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homodimer 2 * 85000, GFP-tagged enzyme, SDS-PAGE Homo sapiens
1.2.1.3 homotetramer 4 * 50000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 aldehyde dehydrogenase 1A1 isoform Homo sapiens
1.2.1.3 ALDH1A1 isoform Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Homo sapiens