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Literature summary extracted from

  • Dai, C.; Han, W.; Wang, M.H.
    Two highly homologous methionine sulfoxide reductase A from tomato (Solanum lycopersicum), exhibit distinct catalytic properties (2012), Protein J., 31, 285-292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 expressed in Escherichia coli BL21(DE3) cells Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 C180S the mutant of isoform E4 shows wild type activity Solanum lycopersicum
1.8.4.11 C180S the mutant of isoform MSRA2 shows strongly reduced catalytic efficiency compared to the wild type enzyme Solanum lycopersicum
1.8.4.11 C188S the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme Solanum lycopersicum
1.8.4.11 C194S the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme Solanum lycopersicum
1.8.4.11 C37S inactive Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 0.00322
-
 thioredoxin wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.00343
-
 thioredoxin mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.0189
-
 thioredoxin mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.0218
-
 thioredoxin wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.02265
-
 thioredoxin mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.0233
-
 thioredoxin mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.11 22000
-
 x * 22000, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin Solanum lycopersicum
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Solanum lycopersicum G3JX11 cultivar Micro-Tom
-
1.8.4.11 Solanum lycopersicum P54153 cultivar Micro-Tom
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.11 fruit
-
 Solanum lycopersicum
-
1.8.4.11 leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Solanum lycopersicum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin
-
 Solanum lycopersicum L-methionine + thioredoxin disulfide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.11 ? x * 22000, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 methionine sulfoxide reductase A
-
 Solanum lycopersicum
1.8.4.11 MSRA2
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.11 0.1
-
 thioredoxin mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.1
-
 thioredoxin mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.11
-
 thioredoxin wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.13
-
 thioredoxin mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.15
-
 thioredoxin wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 0.17
-
 thioredoxin mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 thioredoxin
-
 Solanum lycopersicum

Expression

EC Number Organism Comment Expression
1.8.4.11 Solanum lycopersicum the transcript level of isoform MSRA2 is significantly accumulated in both leaves and green fruits after ethephon treatment up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.11 4.3
-
 thioredoxin mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 4.9
-
 thioredoxin mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 5.1
-
 thioredoxin wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 6.9
-
 thioredoxin mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 46.5
-
 thioredoxin wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum
1.8.4.11 49.5
-
 thioredoxin mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C Solanum lycopersicum