Literature summary extracted from

Tallarita, E.; Pollegioni, L.; Servi, S.; Molla, G.
Expression in Escherichia coli of the catalytic domain of human proline oxidase (2012), Protein Expr. Purif., 82, 345-351.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.5.99.B2	expressed in Escherichia coli BL21(DE3)pLysS cells	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.99.B2	Lactate	the activity is fully inhibited at 5 mM lactate	Homo sapiens
1.5.99.B2	additional information	no inhibition is observed up to 50 mM L-pipecolate	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.99.B2	0.47	-	L-proline	in 10 mM MOPS, pH 7.5, at 25°C	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.5.99.B2	mitochondrial membrane	inner mitochondrial membrane	Homo sapiens	31966	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.5.99.B2	46000	-	2 * 46000, SDS-PAGE	Homo sapiens
1.5.99.B2	46276	-	2 * 46276, calculated from amino acid sequence	Homo sapiens
1.5.99.B2	94900	-	gel filtration	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.99.B2	Homo sapiens	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.99.B2	L-proline + 2,6-dichlorophenolindophenol + H2O	-	Homo sapiens	DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.5.99.B2	homodimer	2 * 46000, SDS-PAGE	Homo sapiens
1.5.99.B2	homodimer	2 * 46276, calculated from amino acid sequence	Homo sapiens

Synonyms

EC Number	Synonyms	Comment	Organism
1.5.99.B2	proline oxidase	-	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.99.B2	FAD	-	Homo sapiens

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Release 2023.1 (January 2023)