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Literature summary extracted from

  • Pandey, B.; Pandey, V.P.; Dwivedi, U.N.
    Cloning, expression, functional validation and modeling of cinnamyl alcohol dehydrogenase isolated from xylem of Leucaena leucocephala (2011), Protein Expr. Purif., 79, 197-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 expression in Escherichia coli Leucaena leucocephala

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.195 homology modeling based on structure of Arabidopsis thaliana isoform CAD5. The model reveals a trilobed structure. The two major lobes contribute to constitution of NADP+ binding, catalytic Zn2+ binding and substrate binding domains. The substrate binding pocket is protected by these two major lobes and is in close proximity of catalytic Zn2+ and NADP+ binding domains Leucaena leucocephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.0122
-
 cinnamyl alcohol pH 8.8, 37°C Leucaena leucocephala
1.1.1.195 0.0181
-
 coniferyl alcohol pH 8.8, 37°C Leucaena leucocephala
1.1.1.195 0.0238
-
 sinapyl alcohol pH 8.8, 37°C Leucaena leucocephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.195 39772
-
 x * 39772, calculated, x * 40000, SDS-PAGE Leucaena leucocephala
1.1.1.195 40000
-
 x * 39772, calculated, x * 40000, SDS-PAGE Leucaena leucocephala

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Leucaena leucocephala B5TR18
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 xylem
-
 Leucaena leucocephala
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 cinnamyl alcohol + NADP+
-
 Leucaena leucocephala cinnamyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 coniferyl alcohol + NADP+
-
 Leucaena leucocephala coniferyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 sinapyl alcohol + NADP+
-
 Leucaena leucocephala sinapyl aldehyde + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.195 ? x * 39772, calculated, x * 40000, SDS-PAGE Leucaena leucocephala

Expression

EC Number Organism Comment Expression
1.1.1.195 Leucaena leucocephala presence of Zn2+ is essential for the expression of enzyme, as no expression is observed in absence of Zn2+ additional information