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Literature summary extracted from

  • Bleve, G.; Lezzi, C.; Spagnolo, S.; Tasco, G.; Tufariello, M.; Casadio, R.; Mita, G.; Rampino, P.; Grieco, F.
    Role of the C-terminus of Pleurotus eryngii Ery4 laccase in determining enzyme structure, catalytic properties and stability (2013), Protein Eng. Des. Sel., 26, 1-13.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 Ery4 genetic structure, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.2 K532A site-directed mutagenesis, the mutant shows increased catalytic efficiency and altered substrate specificity compared to the wild-type enzyme Pleurotus eryngii
1.10.3.2 K532E site-directed mutagenesis, the mutant shows increased catalytic efficiency and altered substrate specificity compared to the wild-type enzyme Pleurotus eryngii
1.10.3.2 K532R site-directed mutagenesis, inactive mutant Pleurotus eryngii
1.10.3.2 additional information construction and generation of C-terminal truncation mutants lacking 1, 2, 5, 8, 11, 14 or 18 amino acid residues Pleurotus eryngii
1.10.3.2 P530A site-directed mutagenesis, the mutant shows increased catalytic efficiency and altered substrate specificity compared to the wild-type enzyme Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information kinetics of wild-type and mutant enzymes, overview Pleurotus eryngii
1.10.3.2 0.058
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 0.084
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 0.105
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 0.653
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 1.219
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 1.278
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 2.174
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 2.487
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 3.703
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant P530A Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Pleurotus eryngii B0JDP9 gene ERY4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae by anion exchange chromatography and gel filtration Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Pleurotus eryngii ?
-
 ?
1.10.3.2 4 2,6-dimethoxyphenol + O2
-
 Pleurotus eryngii ?
-
 ?
1.10.3.2 4 3,3',5,5'-tetramethylbenzidine + O2
-
 Pleurotus eryngii ?
-
 ?
1.10.3.2 4 guaiacol + O2
-
 Pleurotus eryngii ?
-
 ?
1.10.3.2 4 syringaldazine + O2
-
 Pleurotus eryngii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 More enzyme structure modeling, structure comparions, overview Pleurotus eryngii

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Ery4
-
 Pleurotus eryngii
1.10.3.2 Ery4 laccase
-
 Pleurotus eryngii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 4.26
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 4.58
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 5.51
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 60.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 70.02
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 70.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 74.67
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 97.85
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 100.7
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant K532A Pleurotus eryngii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3 6.5 assay at Pleurotus eryngii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 additional information
-
 pH dependencies of wild-type and mutant enzymes, overview Pleurotus eryngii

General Information

EC Number General Information Comment Organism
1.10.3.2 additional information the last 18 amino acids in the C-terminal end of the Ery4 laccase play a critical role in enzyme activity, stability and kinetic and, in particular biochemical and structural data indicate that the K532 residue is fundamental for enzyme activation. Structure-function relationships of fungal laccases, overview Pleurotus eryngii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.074
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 0.133
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 0.154
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 0.672
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 1.165
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 1.73
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 25°C, mutant K532A Pleurotus eryngii
1.10.3.2 2.981
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant P530A Pleurotus eryngii
1.10.3.2 3.505
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant K532E Pleurotus eryngii
1.10.3.2 6.434
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C, mutant K532A Pleurotus eryngii