Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lan, H.-Y.; Liu, C.; Hendry, P.
    Biochemical and enzymatic characterization of a thermostable DNA ligase encoded by thermophilic acidophilic archaebacterium strain JP2 (2006), Prog. Biochem. Biophys., 33, 881-889.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1
-
 uncultured archaeon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Mn2+ the enzyme is most active when Mn2+ is present as divalent metal cofactor rather than Mg2+ and Ca2+ etc. uncultured archaeon

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 uncultured archaeon
-
 acidophilic archaebacterium JP2 from geothermally active sites in Papua New Guinea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1
-
 uncultured archaeon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the donor strans is 42 bp (5'TCCGCGGATCCTGAGGTGAAATGTAAATGAAAAAGCCTGAAC3'), acceptor strand is 38 bp (5'CGTCGAGCAGCGAACCTACTGCGTGGCTTCCGGAGCTA3'), and the complement array stran is 80 bp (5'GTTCAGGCTTTTTCATTTACATTTCACCTCAGGATCCGCGGATAGCTCCGGAAGCCACGCAGTAGGTTCGCTGCTCGACG3') uncultured archaeon AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent DNA ligase
-
 uncultured archaeon

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 50 80 the enzyme displayed relative high activity uncultured archaeon
6.5.1.1 65
-
 assay at uncultured archaeon

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.1 80
-
 5 h, about 5% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene uncultured archaeon
6.5.1.1 85
-
 5 h, about 10% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene uncultured archaeon
6.5.1.1 90
-
 1 h, about 55% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene uncultured archaeon
6.5.1.1 95
-
 10 min, about 80% loss of activity, lysate of cell harbouring with recombinant JP2 ligase gene uncultured archaeon

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 7.4
-
 assay at uncultured archaeon