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Literature summary extracted from

  • Li, Q.; Feng, J.; Gao, C.; Li, F.; Yu, C.; Meng, L.; Zhang, Z.; Ma, C.; Gu, L.; Wu, G.; Xu, P.
    Purification and characterization of a flavin reductase from the biodesulfurizing bacterium Mycobacterium goodii X7B (2012), Process Biochem., 47, 1144-1149.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.36 expressed in Escherichia coli BL21(DE3) cells Mycobacterium goodii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.36 Ag+ strong inhibition at 1 mM Ag+ Mycobacterium goodii
1.5.1.36 Cu2+ strong inhibition at 1 mM Cu2+ Mycobacterium goodii
1.5.1.36 Hg2+ strong inhibition at 1 mM Hg2+ Mycobacterium goodii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.36 0.0066
-
 FMN purified enzyme, at pH 7.0 and 55°C Mycobacterium goodii
1.5.1.36 0.0779
-
 NADH purified enzyme, at pH 7.0 and 55°C Mycobacterium goodii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.36 Cr3+ slight activation by 1 mM Cr3+ Mycobacterium goodii
1.5.1.36 additional information the enzyme is not susceptible to Ca2+, Mg2+, Mn2+ and Co2+ Mycobacterium goodii
1.5.1.36 Zn2+ slight activation by 1 mM Zn2+ Mycobacterium goodii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.36 16989
-
 2 * 16989, calculated from amino acid sequence Mycobacterium goodii
1.5.1.36 17000
-
 2 * 17000, SDS-PAGE Mycobacterium goodii
1.5.1.36 39000
-
 gel filtration Mycobacterium goodii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.36 FMN + NADH + H+ Mycobacterium goodii
-
 FMNH2 + NAD+
-
 ?
1.5.1.36 FMN + NADH + H+ Mycobacterium goodii X7B
-
 FMNH2 + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.36 Mycobacterium goodii
-
-
-
1.5.1.36 Mycobacterium goodii X7B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.36 ammonium sulfate precipitation, butyl-Sepharose column chromatography, Source 30Q column chromatography, FMN-Sepharose 6B affinity column chromatography, and Superdex 75 gel filtration Mycobacterium goodii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.36 0.6
-
 using riboflavin and NADPH as substrates, at pH 7.0 and 55°C Mycobacterium goodii
1.5.1.36 0.8
-
 using FAD and NADPH as substrates, at pH 7.0 and 55°C Mycobacterium goodii
1.5.1.36 1.3
-
 using FMN and NADPH as substrates, at pH 7.0 and 55°C Mycobacterium goodii
1.5.1.36 36
-
 using riboflavin and NADH as substrates, at pH 7.0 and 55°C Mycobacterium goodii
1.5.1.36 189
-
 using FAD and NADH as substrates, at pH 7.0 and 55°C Mycobacterium goodii
1.5.1.36 617
-
 using FMN and NADH as substrates, at pH 7.0 and 55°C Mycobacterium goodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.36 FAD + NADH + H+ the relative activity with FAD is approximately 31% of that of FMN as the acceptor Mycobacterium goodii FADH2 + NAD+
-
 ?
1.5.1.36 FAD + NADH + H+ the relative activity with FAD is approximately 31% of that of FMN as the acceptor Mycobacterium goodii X7B FADH2 + NAD+
-
 ?
1.5.1.36 FMN + NADH + H+
-
 Mycobacterium goodii FMNH2 + NAD+
-
 ?
1.5.1.36 FMN + NADH + H+
-
 Mycobacterium goodii X7B FMNH2 + NAD+
-
 ?
1.5.1.36 additional information less than 0.3% of enzyme activity remains when NADPH is used as the electron donor instead of NADH Mycobacterium goodii ?
-
 ?
1.5.1.36 additional information less than 0.3% of enzyme activity remains when NADPH is used as the electron donor instead of NADH Mycobacterium goodii X7B ?
-
 ?
1.5.1.36 riboflavin + NADH + H+ the relative activity with riboflavin is approximately 5.8% of that of FMN as the acceptor Mycobacterium goodii reduced riboflavin + NAD+
-
 ?
1.5.1.36 riboflavin + NADH + H+ the relative activity with riboflavin is approximately 5.8% of that of FMN as the acceptor Mycobacterium goodii X7B reduced riboflavin + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.36 homodimer 2 * 17000, SDS-PAGE Mycobacterium goodii
1.5.1.36 homodimer 2 * 16989, calculated from amino acid sequence Mycobacterium goodii

Synonyms

EC Number Synonyms Comment Organism
1.5.1.36 DszD
-
 Mycobacterium goodii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.36 55
-
-
 Mycobacterium goodii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.36 45 60 enzyme activity is stable at temperatures below 45°C. After incubation at 60°C for 30 min, more than 90% of the activity is lost Mycobacterium goodii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.36 6.6
-
-
 Mycobacterium goodii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.36 FMN each subunit binds one FMN as cofactor Mycobacterium goodii
1.5.1.36 NADH
-
 Mycobacterium goodii