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Literature summary extracted from

  • Andexer, J.N.; Kendrew, S.G.; Nur-e-Alam, M.; Lazos, O.; Foster, T.A.; Zimmermann, A.S.; Warneck, T.D.; Suthar, D.; Coates, N.J.; Koehn, F.E.; Skotnicki, J.S.; Carter, G.T.; Gregory, M.A.; Martin, C.J.; Moss, S.J.; Leadlay, P.F.; Wilkinson, B.
    Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate (2011), Proc. Natl. Acad. Sci. USA, 108, 4776-4781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.13 expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus subsp. ascomyceticus
4.1.3.45 expressed in Escherichia coli and in Streptomyces hygroscopicus BIOT-4010 cells Streptomyces hygroscopicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.13 0.2
-
 chorismate in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C Streptomyces hygroscopicus subsp. ascomyceticus
4.1.3.45 0.53
-
 chorismate in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.13 chorismate + H2O Streptomyces hygroscopicus subsp. ascomyceticus
-
 (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
 ?
4.1.3.45 chorismate Streptomyces hygroscopicus
-
 3-hydroxybenzoate + pyruvate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.13 Streptomyces hygroscopicus subsp. ascomyceticus
-
-
-
4.1.3.45 Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.13 Ni-NTA column chromatography Streptomyces hygroscopicus subsp. ascomyceticus
4.1.3.45 Ni-NTA column chromatography Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.13 chorismate + H2O
-
 Streptomyces hygroscopicus subsp. ascomyceticus (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
 ?
4.1.3.45 chorismate
-
 Streptomyces hygroscopicus 3-hydroxybenzoate + pyruvate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.13 fkbO
-
 Streptomyces hygroscopicus subsp. ascomyceticus
4.1.3.45 3-hydroxybenzoate synthase
-
 Streptomyces hygroscopicus
4.1.3.45 3HBA synthase
-
 Streptomyces hygroscopicus
4.1.3.45 hyg5
-
 Streptomyces hygroscopicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.13 17.6
-
 chorismate in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C Streptomyces hygroscopicus subsp. ascomyceticus
4.1.3.45 22.4
-
 chorismate in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20°C Streptomyces hygroscopicus